FastQCFastQC Report
Thu 26 May 2016
ERR580158_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580158_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13064523
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT295080.2258635849161887No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247750.18963570273480324No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146620.11222759529758569No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA205450.049.264831
GTATCAA364300.039.202171
CAACGCA417000.032.8419045
ATCAACG421400.032.589583
TATCAAC455600.031.091692
AACGCAG465950.030.0762146
TCAACGC478900.028.872274
GTGGTAT73900.028.5921631
ACGCAGA508950.026.7687427
CGCAGAG519600.026.147628
GGGCACC107300.024.7469694
CTGTGCG118650.024.5571029
GTACATG482400.024.5109671
TAGTACT123050.023.642434
TACATGG488800.023.4896772
GCAGAGT573400.023.3336669
TAGGCAT107000.023.0595235
ACATGGG483400.022.8677673
CTAGTAC135200.022.5959053
CGACGAG45750.021.6767224-25
Produced by FastQC (version 0.11.5)