##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR580158_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13064523
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.517912747369344	34.0	31.0	34.0	31.0	34.0
2	32.72161968714816	34.0	31.0	34.0	31.0	34.0
3	32.802257763257025	34.0	31.0	34.0	31.0	34.0
4	36.276900350667226	37.0	37.0	37.0	35.0	37.0
5	36.20750883901387	37.0	37.0	37.0	35.0	37.0
6	36.239117187822316	37.0	37.0	37.0	35.0	37.0
7	36.20482362808041	37.0	37.0	37.0	35.0	37.0
8	36.198773885583115	37.0	37.0	37.0	35.0	37.0
9	37.98773694225193	39.0	38.0	39.0	35.0	39.0
10-11	37.93625488661163	39.0	38.0	39.0	35.0	39.0
12-13	37.895193150182365	39.0	38.0	39.0	35.0	39.0
14-15	39.29271202630207	41.0	39.0	41.0	36.0	41.0
16-17	39.28342504353202	41.0	39.0	41.0	36.0	41.0
18-19	39.28760460676597	41.0	39.0	41.0	36.0	41.0
20-21	39.27285986637247	41.0	39.0	41.0	36.0	41.0
22-23	39.19698595195554	41.0	39.0	41.0	36.0	41.0
24-25	39.11397105734362	40.5	39.0	41.0	35.0	41.0
26-27	38.975515830160816	40.0	38.5	41.0	35.0	41.0
28-29	38.86883807391973	40.0	38.0	41.0	35.0	41.0
30-31	38.7512544851427	40.0	38.0	41.0	35.0	41.0
32-33	38.60301053471298	40.0	38.0	41.0	35.0	41.0
34-35	38.56833942578692	40.0	38.0	41.0	34.5	41.0
36-37	38.608425466433026	40.0	38.0	41.0	35.0	41.0
38-39	38.50883568424197	40.0	38.0	41.0	34.0	41.0
40-41	38.374510803035065	40.0	38.0	41.0	34.0	41.0
42-43	38.33593702579115	40.0	38.0	41.0	34.0	41.0
44-45	38.22451837698169	40.0	38.0	41.0	34.0	41.0
46-47	38.01158182353845	40.0	37.5	41.0	33.0	41.0
48-49	37.78154265563312	40.0	37.0	41.0	33.0	41.0
50-51	37.602609678133675	40.0	37.0	41.0	33.0	41.0
52-53	37.418260084964444	40.0	36.0	41.0	32.5	41.0
54-55	37.211870383633595	39.5	36.0	41.0	32.0	41.0
56-57	36.99158935997893	39.0	35.0	41.0	32.0	41.0
58-59	36.749157125751935	39.0	35.0	41.0	31.0	41.0
60-61	36.49090085416819	38.5	35.0	40.5	31.0	41.0
62-63	36.20635518036135	38.0	35.0	40.0	31.0	41.0
64-65	35.91702023870293	37.0	35.0	40.0	31.0	41.0
66-67	35.60198129698267	37.0	35.0	40.0	30.0	41.0
68-69	35.2685163093976	36.0	34.5	39.0	30.0	41.0
70-71	34.96471023090548	36.0	34.0	39.0	29.5	41.0
72-73	34.63119824581426	35.0	34.0	38.5	29.0	40.0
74-75	34.28766932401589	35.0	34.0	37.5	29.0	39.5
76-77	33.06139248252692	34.5	32.5	36.0	27.0	39.0
78-79	33.7000458034327	35.0	33.5	36.0	29.0	39.0
80-81	33.64011146828705	35.0	34.0	36.0	29.0	38.5
82-83	33.42992974179003	35.0	34.0	36.0	29.0	37.0
84-85	33.20910958632014	35.0	34.0	35.0	29.0	37.0
86-87	33.02677621678189	35.0	33.5	35.0	29.0	36.5
88-89	32.857876058697286	35.0	33.0	35.0	29.0	36.0
90-91	32.69017636541342	35.0	33.0	35.0	27.5	36.0
92-93	32.56261326953919	35.0	33.0	35.0	27.0	36.0
94-95	32.46143804867579	35.0	33.0	35.0	27.0	35.0
96-97	32.35998727240175	35.0	33.0	35.0	27.0	35.0
98-99	32.28397783830302	35.0	33.0	35.0	27.0	35.0
100	30.980472230023246	34.0	30.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.1270059730268258
1101	2	0.07818594360577436
1101	3	0.028943930423942277
1101	4	0.018980202560456405
1101	5	-0.009866155565013912
1101	6	0.020564323706544485
1101	7	-0.006649747052655641
1101	8	0.006077914517582883
1101	9	0.02625810466216194
1101	10-11	0.004321748036652195
1101	12-13	-0.047132728259249745
1101	14-15	0.010187029656414381
1101	16-17	-0.03293402554920988
1101	18-19	-0.028792555481174986
1101	20-21	-0.024238477798043334
1101	22-23	-0.03978408349451712
1101	24-25	-0.0721022757539842
1101	26-27	-0.0618698008928007
1101	28-29	-0.0932258194139095
1101	30-31	-0.05170224547223512
1101	32-33	-0.08365921558272049
1101	34-35	-0.1389307176487975
1101	36-37	-0.04340830055389233
1101	38-39	-0.013585479837530556
1101	40-41	0.029590641801085837
1101	42-43	-0.022347575349563442
1101	44-45	-0.08345495648910628
1101	46-47	-0.008490445047129924
1101	48-49	-0.044600576780062795
1101	50-51	-0.016747334611409315
1101	52-53	-0.07368403801797996
1101	54-55	-0.0595123495417198
1101	56-57	-0.07508992627727906
1101	58-59	-0.04911716996391391
1101	60-61	-0.12979157383369255
1101	62-63	-0.1363644989119095
1101	64-65	-0.1399148298544688
1101	66-67	-0.14313263269313836
1101	68-69	-0.12286598631057899
1101	70-71	-0.16868293467137363
1101	72-73	-0.13955252012001296
1101	74-75	-0.16688896437682388
1101	76-77	-0.130834788824842
1101	78-79	-0.07274339012624154
1101	80-81	-0.09092517820948132
1101	82-83	-0.08187916068333578
1101	84-85	-0.06953995739642238
1101	86-87	-0.09576536573778327
1101	88-89	-0.10247673791506173
1101	90-91	-0.07774697136283493
1101	92-93	-0.0908902633490527
1101	94-95	-0.16631190095716875
1101	96-97	-0.1242229210918353
1101	98-99	-0.15668349932126802
1101	100	-0.1334378447108442
1102	1	0.08325951363450912
1102	2	0.022741017754498216
1102	3	-0.003159651650065598
1102	4	0.029926265124323947
1102	5	0.00414511171989318
1102	6	0.024236376546461713
1102	7	0.0042310957957525375
1102	8	0.018813069687475092
1102	9	-0.005052844533388168
1102	10-11	0.012102338186082306
1102	12-13	-0.03492262227538845
1102	14-15	0.01908025596114271
1102	16-17	-0.04987969240617929
1102	18-19	-0.029708008664940166
1102	20-21	-0.07252849609735534
1102	22-23	-0.06136985881857271
1102	24-25	-0.0712979398985425
1102	26-27	-0.10826479026320612
1102	28-29	-0.06542039944830691
1102	30-31	-0.06437675240204044
1102	32-33	-0.043579567071880376
1102	34-35	-0.0374688329648194
1102	36-37	-0.004363451210828373
1102	38-39	-0.0012548697625618388
1102	40-41	0.03751209434532399
1102	42-43	0.026860029488688042
1102	44-45	0.01557691474011591
1102	46-47	0.035981416059009064
1102	48-49	0.03824912493421806
1102	50-51	0.01272405288988665
1102	52-53	0.013753535438006281
1102	54-55	-0.01569171034218897
1102	56-57	0.030093017462725413
1102	58-59	-0.03239129964337906
1102	60-61	-0.003350781937122349
1102	62-63	0.004500516879183181
1102	64-65	-0.034042812391682276
1102	66-67	-0.12021582659647834
1102	68-69	-0.016289352108699973
1102	70-71	-0.03580081205367236
1102	72-73	-0.08124231540447369
1102	74-75	-0.06211922105845957
1102	76-77	-0.033198009976615594
1102	78-79	-9.609255499967162E-4
1102	80-81	0.014502308987026424
1102	82-83	0.05528911630077005
1102	84-85	-0.03474694587789173
1102	86-87	-0.021860700382582365
1102	88-89	0.03980068091046718
1102	90-91	0.0056631322804392425
1102	92-93	-0.019432922102069483
1102	94-95	-0.04535474616542956
1102	96-97	-0.10668704628638181
1102	98-99	-0.09117328182997397
1102	100	0.03985093929011185
1103	1	0.1236369370242727
1103	2	0.06877054080783296
1103	3	0.052776513391584956
1103	4	0.004729590693123953
1103	5	-0.012233817422526272
1103	6	0.03710318979152305
1103	7	0.022952823098684405
1103	8	0.0011303213869595652
1103	9	0.01059329626985317
1103	10-11	-0.02953766612584019
1103	12-13	-0.02371379338583779
1103	14-15	-0.021702960306555497
1103	16-17	-0.00955891437547507
1103	18-19	0.0010981281165598489
1103	20-21	-0.04946684820777136
1103	22-23	-0.07723363387223259
1103	24-25	-0.06951592129284734
1103	26-27	-0.06693760064112553
1103	28-29	-0.08787557322675354
1103	30-31	-0.1170169849017455
1103	32-33	-0.13396885757368437
1103	34-35	-0.11549441670003802
1103	36-37	-0.06845858611979594
1103	38-39	-0.07949389915249583
1103	40-41	-0.06436177282305522
1103	42-43	-0.12385890021232626
1103	44-45	-0.06077635705143791
1103	46-47	-0.028043289988367803
1103	48-49	-0.0176293929399165
1103	50-51	-0.022390274786360465
1103	52-53	-0.0596905709651665
1103	54-55	-0.052506737413729354
1103	56-57	-0.040033617516733955
1103	58-59	-0.08429737063543286
1103	60-61	-0.08644910914327397
1103	62-63	-0.1110720704564514
1103	64-65	-0.09069131506199568
1103	66-67	-0.09748234870218653
1103	68-69	-0.07652323223772584
1103	70-71	-0.08221781043553023
1103	72-73	-0.05002249218532029
1103	74-75	-0.11187820062604459
1103	76-77	-0.10495944953667191
1103	78-79	0.0016410646392230888
1103	80-81	-0.009121854349615433
1103	82-83	0.014461984550401041
1103	84-85	-0.015081005470534592
1103	86-87	0.018429643398185647
1103	88-89	-0.011442797424450646
1103	90-91	5.352720719642434E-4
1103	92-93	-0.05241262914183409
1103	94-95	0.0075976446668590825
1103	96-97	-0.018825802146331228
1103	98-99	-0.008545079149818946
1103	100	0.09856277010732484
1104	1	0.09243690277524053
1104	2	0.0465914588964651
1104	3	0.028611481272506012
1104	4	0.008714805074667709
1104	5	-0.00411044447383091
1104	6	0.019070541765898952
1104	7	0.005742599418894656
1104	8	-0.019068221984802847
1104	9	-0.01605242041204491
1104	10-11	-0.027322503366335127
1104	12-13	-0.02076575418011828
1104	14-15	-0.003848303365273864
1104	16-17	-0.050151047853034925
1104	18-19	-0.050562975665165766
1104	20-21	-0.11488950264418207
1104	22-23	-0.11438569101485285
1104	24-25	-0.08532366498247512
1104	26-27	-0.08493865095235975
1104	28-29	-0.14088679638368973
1104	30-31	-0.17882358623013062
1104	32-33	-0.18369116382118733
1104	34-35	-0.13745503479173493
1104	36-37	-0.08607741082828824
1104	38-39	-0.05148889252735955
1104	40-41	-4.584513844960725E-4
1104	42-43	-0.01605959034634452
1104	44-45	-0.10131706820431674
1104	46-47	-0.08323743831662256
1104	48-49	-0.10767101687420677
1104	50-51	-0.09229677459000385
1104	52-53	-0.09559213679661838
1104	54-55	-0.16117165593368554
1104	56-57	-0.11657377223765053
1104	58-59	-0.09740296456141806
1104	60-61	-0.15614207473797848
1104	62-63	-0.1139317755142244
1104	64-65	-0.1167508843861782
1104	66-67	-0.11656889183475982
1104	68-69	-0.09625383561117218
1104	70-71	-0.1265601007969508
1104	72-73	-0.1403727418881786
1104	74-75	-0.18241615544361167
1104	76-77	-0.20546025265002754
1104	78-79	-0.09841587552098474
1104	80-81	-0.08644373112989712
1104	82-83	-0.07913139808707115
1104	84-85	-0.09207637731784502
1104	86-87	-0.07273533207915506
1104	88-89	-0.043729684585315454
1104	90-91	-0.026117049943984227
1104	92-93	-0.06154911743751512
1104	94-95	-0.048717777766640324
1104	96-97	-0.09361286046627271
1104	98-99	-0.08357710870728141
1104	100	-0.004354480551786111
1105	1	0.09900172243151673
1105	2	0.06378520898859819
1105	3	0.04254009182503893
1105	4	0.008017750215266517
1105	5	-0.005006216042396261
1105	6	8.550553897777036E-4
1105	7	-0.007825025972799438
1105	8	-0.005336651695017736
1105	9	-8.653477794240416E-4
1105	10-11	-0.003528063445408236
1105	12-13	0.005240265418706258
1105	14-15	0.037897683785629965
1105	16-17	0.014563671548856405
1105	18-19	-0.0334186807424075
1105	20-21	-0.024542134274852856
1105	22-23	-0.017095160827111044
1105	24-25	-0.02696955672045931
1105	26-27	0.01995660149589895
1105	28-29	-0.021921461538092046
1105	30-31	-0.04493177945733606
1105	32-33	-0.029263004030404716
1105	34-35	-0.04333317621385646
1105	36-37	-0.006582865204521227
1105	38-39	0.0014295983586976035
1105	40-41	-0.0060958059636035955
1105	42-43	-0.002739038471744948
1105	44-45	0.0043152136791704265
1105	46-47	-0.0562179201446682
1105	48-49	-0.012779467060191507
1105	50-51	0.010400974549440889
1105	52-53	-0.01794530971715602
1105	54-55	0.02343628250257268
1105	56-57	0.005092736900863315
1105	58-59	0.04886711558139467
1105	60-61	0.04070802696161735
1105	62-63	-0.034365157146559966
1105	64-65	0.026877477503113312
1105	66-67	0.00970918900407014
1105	68-69	0.06069692802462612
1105	70-71	0.019744043002845046
1105	72-73	0.029757145133778806
1105	74-75	-0.043553959628304995
1105	76-77	-0.01293718922745768
1105	78-79	0.011030784434112206
1105	80-81	0.031178827942440535
1105	82-83	0.07798114262816824
1105	84-85	0.06901477540960599
1105	86-87	0.07253336037067726
1105	88-89	0.026807795698374548
1105	90-91	0.053576443622944225
1105	92-93	0.07003679509418248
1105	94-95	0.028262286105970702
1105	96-97	0.043948080528885214
1105	98-99	0.05626116913133927
1105	100	0.06272965017750565
1106	1	0.10175104457572104
1106	2	0.09231648258865022
1106	3	0.03378711408750945
1106	4	0.011669461270386705
1106	5	0.011520032468894215
1106	6	0.009437324174726314
1106	7	0.028445741450681794
1106	8	0.03551939666665049
1106	9	0.02567108614581315
1106	10-11	0.018310290196922097
1106	12-13	-0.010906148803115912
1106	14-15	0.01770200842217662
1106	16-17	0.005158951271205581
1106	18-19	-0.0025583440469532093
1106	20-21	-0.006708679196577805
1106	22-23	-0.015324826490555665
1106	24-25	0.010655010400910214
1106	26-27	-0.06848215997811025
1106	28-29	-0.05287715073389876
1106	30-31	-0.04995725117770888
1106	32-33	-0.04558150704907149
1106	34-35	0.009139263516551921
1106	36-37	-0.018837949060902304
1106	38-39	-0.023517681797251555
1106	40-41	-0.024323627547055082
1106	42-43	-0.04712887967242807
1106	44-45	-0.03215533882187316
1106	46-47	-0.03463821129238198
1106	48-49	-0.014309018573676724
1106	50-51	-0.01790955027987451
1106	52-53	-0.011436189692069831
1106	54-55	0.013933587435786876
1106	56-57	-0.0011919567177258727
1106	58-59	-0.046062865946517206
1106	60-61	-0.029807077962523465
1106	62-63	-0.07541963997551449
1106	64-65	-0.02532556809966735
1106	66-67	-0.04381788147928489
1106	68-69	-0.021824973567987627
1106	70-71	-0.024129990780942023
1106	72-73	-0.07885286378287759
1106	74-75	-0.07075404309718891
1106	76-77	-0.09952733066705122
1106	78-79	-0.017754631921754083
1106	80-81	-0.025692444194156394
1106	82-83	0.005676955610631751
1106	84-85	0.031522793196380405
1106	86-87	0.04498968106449297
1106	88-89	0.054366910550498915
1106	90-91	0.0234259695602006
1106	92-93	-0.04867473791015442
1106	94-95	0.04163620440088778
1106	96-97	0.0564014322188271
1106	98-99	0.04852520385032477
1106	100	0.023493135545994193
1107	1	0.10931465625068881
1107	2	0.06142717468259207
1107	3	0.031623920003163164
1107	4	0.03177127110270561
1107	5	0.02129153958552621
1107	6	0.013760201975493658
1107	7	0.027593523759996685
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2315	100	-0.17695517530822968
2316	1	0.07417383055056348
2316	2	-0.019920828001097846
2316	3	-0.01920755877087288
2316	4	-0.140579668595187
2316	5	-0.0857772248670372
2316	6	-0.03883927768227835
2316	7	-0.0015088822029554194
2316	8	0.011951155415268033
2316	9	0.013733638081411925
2316	10-11	0.001640698250305661
2316	12-13	0.002801828054586508
2316	14-15	0.002524114842117342
2316	16-17	0.013873623223659592
2316	18-19	0.012223891514729246
2316	20-21	0.0031564426630694697
2316	22-23	-0.04231130083202572
2316	24-25	-0.03556276301250705
2316	26-27	0.0300174511383986
2316	28-29	0.020870963103156726
2316	30-31	-0.2640800993702399
2316	32-33	-0.10570547649270878
2316	34-35	-0.14851988999222243
2316	36-37	-0.36988924456030503
2316	38-39	-0.2536635254442672
2316	40-41	-0.2072044318989157
2316	42-43	-0.3017252467155842
2316	44-45	-0.142950511238368
2316	46-47	-0.30191722486098627
2316	48-49	-0.2573763103052258
2316	50-51	-0.3011740064198989
2316	52-53	-0.2851911936245415
2316	54-55	-0.14588731663307186
2316	56-57	-0.8454371080729643
2316	58-59	-0.6568273545146539
2316	60-61	-0.37323776809022746
2316	62-63	-0.1346857739096663
2316	64-65	-0.07887798281430491
2316	66-67	-0.03424502300534016
2316	68-69	-0.12770495204803467
2316	70-71	-0.14578276336689555
2316	72-73	-0.09070616887238003
2316	74-75	-0.0354399091262394
2316	76-77	-0.07188413718758113
2316	78-79	-0.1211488811723882
2316	80-81	-0.12911875974039333
2316	82-83	-0.24003272200278758
2316	84-85	-0.03500418851769638
2316	86-87	-0.1453101937732697
2316	88-89	-0.17953180212003872
2316	90-91	-0.14301986736142425
2316	92-93	-0.14974838747333052
2316	94-95	-0.08636625363349992
2316	96-97	-0.2390534301283651
2316	98-99	-0.08984917525940972
2316	100	-0.34979644376440433
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	14.0
13	146.0
14	492.0
15	1628.0
16	4075.0
17	9198.0
18	16916.0
19	26495.0
20	40038.0
21	58946.0
22	77162.0
23	78440.0
24	73470.0
25	73849.0
26	83408.0
27	100966.0
28	124995.0
29	154480.0
30	189430.0
31	236222.0
32	299084.0
33	393307.0
34	583309.0
35	895586.0
36	1618924.0
37	3257107.0
38	3736886.0
39	929751.0
40	199.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.38985747053895	20.202997459497563	13.72953638845529	23.677608681508193
2	15.549223181042516	20.029287147074264	40.42854057052912	23.992949101354107
3	18.614985228790136	25.71102928484687	29.92990420815867	25.744081278204323
4	12.696380263744263	16.852309672097796	38.8800276049534	31.571282459204543
5	13.301202118933258	37.99662306876674	34.91769552995223	13.784479282347764
6	30.760219871785598	37.67486956852539	17.651957136131184	13.912953423557827
7	27.092493007010138	32.40900999593402	22.56036811784474	17.938128879211103
8	26.00525669843271	35.27439374903268	19.90147479339773	18.81887475913688
9	26.047784491759984	15.56496793926997	21.04777415810779	37.33947341086226
10-11	25.29363607760825	26.035615486179413	27.757508919103646	20.913239517108693
12-13	25.62118259872773	23.718574802158763	28.097631018248208	22.5626115808653
14-15	22.98061329209688	25.14990536371773	25.711729713190163	26.157751630995236
16-17	22.17296874902527	27.98140947481016	26.705180437574192	23.140441338590374
18-19	21.744224100414613	27.576481066648828	28.844729761086878	21.834565071849678
20-21	22.98564663399007	26.70202024241807	28.449523485271705	21.86280963832016
22-23	23.056064440879567	26.50825113731851	28.1759036815946	22.25978074020732
24-25	22.655783146464668	26.57869330552673	28.600745698867076	22.164777849141526
26-27	22.5949509907407	26.813324223496366	28.022687994870893	22.569036790892042
28-29	22.103090420663555	27.080624042913016	28.33840207314112	22.477883463282307
30-31	22.74037789370472	26.701237566040387	28.30444002885624	22.25394451139865
32-33	22.76595552481142	26.941963708140783	27.832517802278154	22.459562964769642
34-35	22.826075598556095	26.93705956128667	28.266600719794138	21.970264120363094
36-37	22.689594777168953	26.65975416221047	28.047641851377886	22.60300920924269
38-39	22.704672849404208	26.71518834138596	28.352296310858407	22.22784249835143
40-41	22.61296826837318	26.19872680617193	28.668690526811858	22.519614398643036
42-43	22.482311363406808	26.352953973509845	28.25708001881705	22.907654644266298
44-45	22.59258234418205	26.889663490122917	28.072343249359434	22.445410916335604
46-47	22.57122262513139	26.727184565122762	27.652229872788713	23.049362936957134
48-49	23.054896049070038	26.667958120457808	27.672501710582985	22.60464411988917
50-51	22.835054527345157	26.7184163491826	27.46024268217458	22.986286441297665
52-53	22.498288874956383	27.01653545968169	27.375363634404714	23.10981203095721
54-55	22.447754446453295	26.910244377628967	27.412864625781136	23.2291365501366
56-57	22.99560553424909	26.702031202800292	27.138867651312044	23.163495611638577
58-59	22.41187667355624	26.961067769304176	27.608114627252274	23.01894092988731
60-61	22.53160078683642	27.224562284161724	27.211549891541708	23.032287037460154
62-63	22.68114107747404	26.84771174526417	27.062088399235513	23.40905877802628
64-65	22.20098398069147	26.863238532803894	27.275830411671826	23.659947074832807
66-67	22.57398285180007	26.722529908694238	27.18170333070659	23.5217839087991
68-69	22.31059825684679	26.673318885068092	27.385457337654977	23.63062552043014
70-71	22.296528394114855	26.433663505024906	27.38237800927175	23.887430091588488
72-73	22.558387888758716	26.54438453967249	27.39771529904275	23.499512272526047
74-75	22.593926304509363	26.770857884858383	27.213950795624424	23.42126501500783
76-77	22.38734599565058	26.733123145649028	26.989858457627786	23.88967240107261
78-79	22.285458933674068	26.71617276280115	27.07412488536523	23.92424341815956
80-81	22.089039385456164	27.05691998085339	27.22919659600024	23.624844037690206
82-83	22.54704670288928	26.46055148797793	27.779630764810097	23.212771044322697
84-85	22.66717721763881	26.636290031989805	27.102288351458082	23.5942443989133
86-87	21.868753187932544	27.078313860544164	27.350716919550944	23.70221603197235
88-89	22.326061043755253	26.323545030193884	27.337486248324872	24.012907677725988
90-91	22.264196450563965	26.36255905125478	27.33866238105743	24.034582117123826
92-93	22.31525336273262	26.49162733130727	27.27263193979515	23.92048736616496
94-95	21.672151162345664	26.410759256282486	27.664653516124343	24.252436065247508
96-97	21.85558903696249	26.320363610783865	27.45727880232992	24.36676854992373
98-99	21.47709969269588	26.6115746192072	27.38495193412071	24.52637375397621
100	21.87318922948916	26.291564957337577	26.99211355621742	24.843132256955847
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	857.0
1	611.0
2	472.5
3	582.0
4	498.5
5	552.5
6	962.0
7	1838.0
8	3101.5
9	3963.5
10	3763.0
11	3534.0
12	4023.0
13	4814.5
14	5744.0
15	6901.0
16	8303.5
17	9635.0
18	11028.0
19	12271.5
20	13487.0
21	15060.0
22	17002.5
23	19829.5
24	23727.5
25	29410.0
26	38546.5
27	49594.5
28	63463.5
29	85073.5
30	113140.0
31	141549.5
32	179109.5
33	217115.0
34	249534.0
35	284997.0
36	322602.0
37	372483.5
38	400255.5
39	399938.0
40	392027.0
41	383711.5
42	392955.0
43	414353.5
44	441907.5
45	492902.5
46	537232.0
47	564306.0
48	587605.0
49	584445.5
50	565067.5
51	558171.5
52	547834.0
53	522753.5
54	497765.0
55	444928.0
56	392623.0
57	333811.0
58	272794.5
59	244399.0
60	200998.5
61	151383.5
62	117799.0
63	92970.5
64	67693.5
65	41538.0
66	27320.5
67	20028.0
68	15036.5
69	11283.0
70	8373.5
71	6161.5
72	4428.0
73	3010.5
74	2121.5
75	1447.5
76	967.0
77	584.0
78	324.0
79	196.0
80	112.0
81	63.0
82	43.0
83	31.0
84	22.5
85	15.0
86	9.5
87	9.0
88	6.5
89	3.5
90	1.0
91	1.0
92	1.5
93	0.5
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3538743817895227
2	6.812342096225022E-4
3	0.001974813776209051
4	0.0015385177093721679
5	0.004523701324571896
6	0.0
7	8.419748658255644E-5
8	3.7506153114047865E-4
9	0.00313826995444074
10-11	7.271601113948056E-5
12-13	5.35802187343541E-5
14-15	2.3345666734254285E-4
16-17	1.4160486379793583E-4
18-19	9.912340465855509E-4
20-21	2.0283939949434048E-4
22-23	1.607406562030623E-4
24-25	0.0
26-27	0.0019671594592470007
28-29	0.011075796644087197
30-31	0.00346357842532789
32-33	0.014305918402072543
34-35	0.011347524896239993
36-37	0.013184560967132133
38-39	0.013019993152448046
40-41	0.014248511024857163
42-43	0.006425799089641466
44-45	0.0012744437741814224
46-47	0.006004811656728684
48-49	0.013203696759537259
50-51	0.003685553617227357
52-53	0.005736910563056914
54-55	0.0052355528020426
56-57	1.7222213164613817E-4
58-59	6.92715685065578E-4
60-61	4.133331159507316E-4
62-63	0.0010256784729147784
64-65	2.9851836151997287E-4
66-67	0.0013624684192450043
68-69	0.0016035794035495975
70-71	8.266662319014632E-4
72-73	0.0027746898987433375
74-75	0.0077308601316710916
76-77	0.0037085165681135087
78-79	0.0015767892941824205
80-81	5.243207119004651E-4
82-83	2.4111098430459345E-4
84-85	0.0015729621357013952
86-87	0.004665306188369831
88-89	0.0015729621357013952
90-91	1.9135792405126461E-4
92-93	0.001986295251652127
94-95	0.003237776074947397
96-97	0.0017145669994993312
98-99	0.002036048311905456
100	0.002548887548362845
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.3064523E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.91888749504066
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.06889589301645	35.34061347781156
2	16.243489406094863	16.866877980038478
3	6.496296058504751	10.118413925879526
4	3.3054871091029607	6.864688533352951
5	1.8369601468891776	4.768646359960628
6	1.091980328859126	3.401664230450068
7	0.7221394587290317	2.6244874119437505
8	0.46530246954123167	1.932638925382063
9	0.3420087561933053	1.5981042721607104
>10	1.3589795271951124	11.51409200348063
>50	0.0470550180615136	1.6592874675092142
>100	0.01985264008724713	1.802932415711581
>500	0.0010291150840105938	0.3590828356231251
>1k	4.4344623853266894E-4	0.36279360167556
>5k	2.015660069243183E-5	0.0641614228695286
>10k+	6.0469802077295484E-5	0.7215151361506524
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29508	0.2258635849161887	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24775	0.18963570273480324	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14662	0.11222759529758569	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.654316962050585E-6	0.0
2	0.0	0.0	0.0	7.654316962050585E-6	0.0
3	0.0	0.0	0.0	7.654316962050585E-6	0.0
4	0.0	0.0	0.0	1.530863392410117E-5	2.2962950886151755E-5
5	0.0	4.592590177230351E-5	0.0	1.530863392410117E-5	2.2962950886151755E-5
6	1.8370360708921404E-4	4.592590177230351E-5	0.0	1.530863392410117E-5	2.2962950886151755E-5
7	1.8370360708921404E-4	6.123453569640468E-5	0.0	1.530863392410117E-5	2.2962950886151755E-5
8	1.9135792405126464E-4	6.123453569640468E-5	0.0	1.530863392410117E-5	2.2962950886151755E-5
9	1.9135792405126464E-4	6.123453569640468E-5	0.0	2.2962950886151755E-5	3.061726784820234E-5
10-11	1.9901224101331522E-4	6.506169417742997E-5	0.0	3.061726784820234E-5	3.061726784820234E-5
12-13	2.066665579753658E-4	7.654316962050585E-5	0.0	3.061726784820234E-5	3.061726784820234E-5
14-15	2.066665579753658E-4	8.419748658255644E-5	0.0	4.9753060253328805E-5	3.444442632922763E-5
16-17	2.449381427856187E-4	8.419748658255644E-5	0.0	6.123453569640468E-5	3.8271584810252924E-5
18-19	2.449381427856187E-4	9.950612050665761E-5	0.0	8.037032810153115E-5	3.8271584810252924E-5
20-21	2.525924597476693E-4	1.071604374687082E-4	0.0	1.4160486379793583E-4	3.8271584810252924E-5
22-23	2.525924597476693E-4	1.071604374687082E-4	0.0	3.4444426329227634E-4	4.592590177230351E-5
24-25	2.564196182286946E-4	1.1098759594973349E-4	0.0	8.075304394963368E-4	5.35802187343541E-5
26-27	2.602467767097199E-4	1.1481475443075878E-4	0.0	0.0016112337205116483	6.123453569640468E-5
28-29	2.602467767097199E-4	1.2246907139280936E-4	0.0	0.004787775259762641	6.123453569640468E-5
30-31	2.6407393519074524E-4	1.3012338835485996E-4	0.0	0.01676295414689078	6.123453569640468E-5
32-33	2.679010936717705E-4	1.3395054683588525E-4	0.0	0.04480837149584413	6.888885265845527E-5
34-35	2.8320972759587166E-4	1.492591807599864E-4	0.0	0.08831933626662068	6.888885265845527E-5
36-37	2.8320972759587166E-4	1.7604929012716346E-4	0.0	0.15857065734432096	6.888885265845527E-5
38-39	2.8320972759587166E-4	1.9901224101331522E-4	0.0	0.25968418441300917	6.888885265845527E-5
40-41	2.870368860768969E-4	2.104937164563911E-4	0.0	0.366634128165261	6.888885265845527E-5
42-43	2.9086404455792224E-4	2.143208749374164E-4	0.0	0.4760066632360018	6.888885265845527E-5
44-45	2.9469120303894755E-4	2.2962950886151756E-4	0.0	0.6089965932931497	6.888885265845527E-5
46-47	3.1382699544407397E-4	2.4111098430459343E-4	0.0	0.7671271274121527	7.654316962050585E-5
48-49	3.291356293681752E-4	2.449381427856187E-4	0.0	0.9362186434208122	7.654316962050585E-5
50-51	3.291356293681752E-4	2.48765301266644E-4	0.0	1.109902749606702	7.654316962050585E-5
52-53	3.291356293681752E-4	2.525924597476693E-4	0.0	1.28029549949891	8.419748658255644E-5
54-55	3.3678994633022576E-4	3.3678994633022576E-4	0.0	1.4664331793820562	9.185180354460702E-5
56-57	3.4444426329227634E-4	3.9037016506457986E-4	0.0	1.6830388679326447	9.185180354460702E-5
58-59	3.4444426329227634E-4	4.133331159507316E-4	0.0	1.9261093573795232	9.185180354460702E-5
60-61	3.4444426329227634E-4	4.171602744317569E-4	0.0	2.177059966138833	9.185180354460702E-5
62-63	3.6740721417842807E-4	4.248145913938075E-4	0.0	2.432802177316386	9.950612050665761E-5
64-65	3.941973235456051E-4	4.592590177230351E-4	0.0	2.695532779880291	9.950612050665761E-5
66-67	3.9802448202663044E-4	4.745676516471363E-4	0.0	2.9872081820361904	9.950612050665761E-5
68-69	3.9802448202663044E-4	4.745676516471363E-4	0.0	3.3127156651643537	9.950612050665761E-5
70-71	3.9802448202663044E-4	4.898762855712374E-4	0.0	3.662808814374624	9.950612050665761E-5
72-73	3.9802448202663044E-4	5.051849194953386E-4	0.0	4.017284060045667	1.2246907139280936E-4
74-75	4.0185164050765576E-4	5.051849194953386E-4	0.0	4.366477827012896	1.2246907139280936E-4
76-77	4.05678798988681E-4	5.051849194953386E-4	0.0	4.753277253214679	1.2246907139280936E-4
78-79	4.05678798988681E-4	5.128392364573892E-4	0.0	5.172041107049986	1.2246907139280936E-4
80-81	4.095059574697063E-4	5.281478703814904E-4	0.0	5.62039655026058	1.2246907139280936E-4
82-83	4.133331159507316E-4	5.281478703814904E-4	0.0	6.079184827490449	1.530863392410117E-4
84-85	4.133331159507316E-4	5.281478703814904E-4	0.0	6.5298480472651015	1.530863392410117E-4
86-87	4.209874329127822E-4	5.434565043055915E-4	0.0	7.000002985183615	1.6456781468408756E-4
88	4.209874329127822E-4	5.664194551917433E-4	0.0	7.375340071734728	1.8370360708921404E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	20545	0.0	49.26483	1
GTATCAA	36430	0.0	39.20217	1
CAACGCA	41700	0.0	32.841904	5
ATCAACG	42140	0.0	32.58958	3
TATCAAC	45560	0.0	31.09169	2
AACGCAG	46595	0.0	30.076214	6
TCAACGC	47890	0.0	28.87227	4
GTGGTAT	7390	0.0	28.592163	1
ACGCAGA	50895	0.0	26.768742	7
CGCAGAG	51960	0.0	26.14762	8
GGGCACC	10730	0.0	24.74696	94
CTGTGCG	11865	0.0	24.557102	9
GTACATG	48240	0.0	24.510967	1
TAGTACT	12305	0.0	23.64243	4
TACATGG	48880	0.0	23.489677	2
GCAGAGT	57340	0.0	23.333666	9
TAGGCAT	10700	0.0	23.059523	5
ACATGGG	48340	0.0	22.867767	3
CTAGTAC	13520	0.0	22.595905	3
CGACGAG	4575	0.0	21.67672	24-25
>>END_MODULE