FastQCFastQC Report
Thu 26 May 2016
ERR580157_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580157_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6066357
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146100.24083646907031683No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107030.17643208271455174No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68570.11303324219131844No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93200.047.07921
GTATCAA175350.042.5364041
CAACGCA197350.036.0862465
ATCAACG201050.035.44643
TATCAAC222050.033.4963262
GTACTAG50350.032.3176651
AACGCAG228850.032.026586
GTGGTAT26100.030.0911881
ACGCAGA239150.029.6062477
TCAACGC246100.029.3402444
CGCAGAG242950.028.930458
GGGCACC55200.028.52921994
GCATCGT51650.028.3808088
TAGGCAT64150.028.2117675
CTGTGCG66950.027.6507959
TAGTACT66700.027.6276784
GTACATG274650.026.9004751
TATTCTC28450.026.7669545
CGTTAAC13000.026.4084471
GCAGAGT261350.026.3915889
Produced by FastQC (version 0.11.5)