FastQCFastQC Report
Thu 26 May 2016
ERR580157_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580157_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6066357
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128930.21253282653823374No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99180.16349186175492145No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65850.10854949683970132No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92650.046.7887461
GTATCAA174200.042.698661
CAACGCA198500.035.7552535
ATCAACG203650.034.921453
TATCAAC219650.033.6063082
AACGCAG234950.031.3846476
GTACTAG53200.030.5787981
GGGCACC57000.028.77628194
ACGCAGA245300.028.7384597
TCAACGC252000.028.519454
CGCAGAG250800.028.0328128
TAGGCAT64950.027.93395
TAGTACT65000.027.406914
CTGTGCG66200.026.905679
GCTATTG60700.026.6337538
CTAGGCA71600.025.996594
GTACATG273050.025.6910931
AGGGCTA63350.025.67165
CTATTGA63600.025.4932129
GCAGAGT272000.025.4332289
Produced by FastQC (version 0.11.5)