FastQCFastQC Report
Thu 26 May 2016
ERR580156_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580156_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8984371
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204070.22713888373487695No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163760.18227208115070048No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100170.11149361485628766No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA134600.044.1350061
GTATCAA228000.040.372061
CAACGCA258150.034.1032455
ATCAACG268000.032.9074173
TATCAAC291100.031.4276542
GTACTAG67750.030.8941041
AACGCAG294550.030.621326
GTACATG349900.028.8476661
TCAACGC311100.028.619434
TACATGG355900.028.1505072
ACGCAGA318450.027.6437667
ACATGGG354000.027.238173
GTGGTAT47000.026.9202631
CGCAGAG330200.026.6031428
TAGTACT94800.026.444134
GCATCGT71550.026.341198
GGGCACC85350.026.10050694
CTGTGCG95500.025.8390169
CATGGGG295800.025.7747944
TAGGCAT85350.025.4566045
Produced by FastQC (version 0.11.5)