FastQCFastQC Report
Thu 26 May 2016
ERR580156_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580156_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8984371
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181500.20201748124604382No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151100.16818094444229875No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94190.10483761189291939No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA133150.043.461981
GTATCAA225700.039.449461
CAACGCA253100.033.796395
ATCAACG263000.032.4711383
TATCAAC291950.030.282262
AACGCAG290300.030.2415476
GTACTAG61350.030.0375231
TCAACGC300200.028.7287334
GTACATG327400.028.4875831
TACATGG331750.027.4143562
ACGCAGA313950.027.14017
ACATGGG327500.026.7075083
GTGGTAT44750.026.2627121
CGCAGAG326050.026.162038
TAGGCAT83150.025.1529465
CATGGGG272300.025.0961914
CTAACGC29900.024.9933383
GGGCACC81650.024.58466394
ATGGGGG215550.024.1810195
CTGTGCG93700.024.0256379
Produced by FastQC (version 0.11.5)