FastQCFastQC Report
Thu 26 May 2016
ERR580155_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580155_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6011973
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196210.3263654045019829No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164570.2737370909683061No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97480.16214311009048113No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91800.051.625991
GTATCAA169550.041.844851
CAACGCA203450.034.0621645
ATCAACG204100.034.0475273
TATCAAC215400.032.7203032
TCAACGC214850.032.3428844
AACGCAG220200.031.9692046
ACGCAGA243800.028.3341457
CGCAGAG251250.027.3252038
GTACATG195350.026.3061491
GTGGTAT41400.025.7795941
TACATGG199350.025.1657312
GCAGAGT274500.024.467659
ACATGGG200900.024.4562853
GTACTAG27350.023.5512391
CATGGGG151850.023.2212834
TGGTATC44600.023.0871682
TAACGCC12200.021.966194
CTAACGC12150.021.2833773
GAGTACT172000.021.0041212-13
Produced by FastQC (version 0.11.5)