FastQCFastQC Report
Thu 26 May 2016
ERR580155_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580155_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6011973
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173940.2893226566386775No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154600.2571535168238447No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94790.1576687054316445No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92750.054.6333271
GTATCAA167350.041.2463841
ATCAACG208150.032.061623
CAACGCA210000.031.8233995
TATCAAC218600.031.2381532
TCAACGC220800.030.3734954
AACGCAG224600.030.089286
GTGGTAT37600.027.2294671
ACGCAGA251350.026.5878587
CGCAGAG257400.025.7986098
GTACATG189500.024.623761
GTACTAG27400.023.7627011
TACATGG195000.023.7394962
TGGTATC41400.023.2714482
GCAGAGT283950.023.0055149
ACATGGG197750.022.4826833
AGAGTAC262500.020.6418310-11
GAGTACT182000.020.36020512-13
CATGGGG150750.020.2328594
CAGAGTA278650.019.9008310-11
Produced by FastQC (version 0.11.5)