FastQCFastQC Report
Thu 26 May 2016
ERR580154_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580154_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7721692
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110510.1431163014531012No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86170.11159471266142189No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA121900.044.4365771
GTATCAA214300.039.582771
CAACGCA225550.034.7906955
ATCAACG233400.033.6034773
TATCAAC257850.032.3685342
AACGCAG269850.030.0920286
ACGCAGA273900.028.309577
CGCAGAG276400.027.9851448
TCAACGC289500.027.4966964
GTACTAG66500.027.2226851
GCATCGT62850.025.197678
GCAGAGT305300.024.8165539
GTGATCG42050.024.5862568
GGGCACC65800.024.56664394
TAGGCAT84800.024.2289855
GTATAAG63000.023.6597711
TACCCCG7400.023.5082135
GTGGTAT43250.023.3746011
GTACATG343850.023.2335851
CTGTGCG78350.022.9154119
Produced by FastQC (version 0.11.5)