FastQCFastQC Report
Thu 26 May 2016
ERR580154_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580154_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7721692
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95440.12359985350361034No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80540.10430356455554042No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA121000.043.6515961
GTATCAA214250.039.7965161
CAACGCA230450.035.076615
ATCAACG242150.033.440683
TATCAAC264800.031.892842
AACGCAG277600.030.4043626
ACGCAGA286700.028.2427257
TCAACGC294950.027.9001464
CGCAGAG290150.027.8259268
GTACTAG63850.026.0724161
GCAGAGT320600.024.7581399
CGTTAAC20700.024.3770371
GTGGTAT42850.023.5521431
GGGCACC70000.023.4309894
TAGGCAT83400.023.3292185
CTGTGCG80550.022.1118729
GAGTACT116050.021.98905212-13
GTGATCG42450.021.9197988
GTACATG349800.021.6787221
TACATGG354100.021.0892472
Produced by FastQC (version 0.11.5)