##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR580154_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7721692
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.548279962474545	34.0	31.0	34.0	31.0	34.0
2	32.748110129230746	34.0	31.0	34.0	31.0	34.0
3	32.83133709554849	34.0	31.0	34.0	31.0	34.0
4	36.294202099747054	37.0	37.0	37.0	35.0	37.0
5	36.23180489457492	37.0	37.0	37.0	35.0	37.0
6	36.25752257406796	37.0	37.0	37.0	35.0	37.0
7	36.22783180163104	37.0	37.0	37.0	35.0	37.0
8	36.221686904890795	37.0	37.0	37.0	35.0	37.0
9	38.00514161922025	39.0	38.0	39.0	35.0	39.0
10-11	37.95593957904563	39.0	38.0	39.0	35.0	39.0
12-13	37.915591492123745	39.0	38.0	39.0	35.0	39.0
14-15	39.317069432450815	41.0	39.0	41.0	36.0	41.0
16-17	39.30501728118656	41.0	39.0	41.0	36.0	41.0
18-19	39.300143737926874	41.0	39.0	41.0	36.0	41.0
20-21	39.28149847209653	41.0	39.0	41.0	36.0	41.0
22-23	39.20997774840022	41.0	39.0	41.0	36.0	41.0
24-25	39.12129828540169	40.5	39.0	41.0	35.0	41.0
26-27	38.98142233593363	40.0	38.5	41.0	35.0	41.0
28-29	38.887301189946456	40.0	38.0	41.0	35.0	41.0
30-31	38.78857263408072	40.0	38.0	41.0	35.0	41.0
32-33	38.66103420079433	40.0	38.0	41.0	35.0	41.0
34-35	38.64021816720999	40.0	38.0	41.0	35.0	41.0
36-37	38.693940783962894	40.0	38.0	41.0	35.0	41.0
38-39	38.604869502694484	40.0	38.0	41.0	35.0	41.0
40-41	38.486638226440526	40.0	38.0	41.0	34.0	41.0
42-43	38.46335738332998	40.0	38.0	41.0	34.0	41.0
44-45	38.3681968278455	40.0	38.0	41.0	34.0	41.0
46-47	38.18220740998217	40.0	37.5	41.0	34.0	41.0
48-49	37.968338092221245	40.0	37.0	41.0	33.0	41.0
50-51	37.781268729703285	40.0	36.5	41.0	33.0	41.0
52-53	37.58776871701176	40.0	36.0	41.0	33.0	41.0
54-55	37.37234999790201	39.0	36.0	41.0	33.0	41.0
56-57	37.127132434186706	39.0	35.0	41.0	32.0	41.0
58-59	36.8656242699139	39.0	35.0	41.0	32.0	41.0
60-61	36.592669909651924	38.0	35.0	40.0	32.0	41.0
62-63	36.2885486108485	37.0	35.0	40.0	31.0	41.0
64-65	35.996348857219374	37.0	35.0	40.0	31.0	41.0
66-67	35.669341447444424	36.0	35.0	39.5	31.0	41.0
68-69	35.32881096526512	36.0	35.0	39.0	30.5	41.0
70-71	35.03198897340117	35.0	34.0	38.5	30.0	40.0
72-73	34.70441743856139	35.0	34.0	37.5	30.0	40.0
74-75	34.370743873234005	35.0	34.0	37.0	30.0	39.0
76-77	33.16673042644021	34.5	32.5	36.0	28.0	38.5
78-79	33.801353770650266	35.0	34.0	36.0	29.0	38.5
80-81	33.756084417767504	35.0	34.0	36.0	30.0	37.0
82-83	33.56908032591821	35.0	34.0	35.5	29.0	37.0
84-85	33.37688792818983	35.0	34.0	35.0	29.0	36.5
86-87	33.23434429915102	35.0	34.0	35.0	29.0	36.0
88-89	33.09957409593649	35.0	34.0	35.0	29.0	36.0
90-91	32.953412412719906	35.0	33.0	35.0	29.0	36.0
92-93	32.850642579372504	35.0	33.0	35.0	29.0	35.0
94-95	32.7725865652243	35.0	33.0	35.0	29.0	35.0
96-97	32.6820101086653	35.0	33.0	35.0	29.0	35.0
98-99	32.62044309718647	35.0	33.0	35.0	29.0	35.0
100	31.324574588056606	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.1775181229653171
1101	2	0.09838517433626492
1101	3	0.042638170559591515
1101	4	0.049902318700659976
1101	5	0.003771994440285198
1101	6	0.05740820635999455
1101	7	0.014292234091087153
1101	8	0.017838124627822083
1101	9	0.013101192477599
1101	10-11	0.04056779500370311
1101	12-13	0.028192037576808104
1101	14-15	0.021955644051026013
1101	16-17	-0.002894995234861142
1101	18-19	0.020671348411831048
1101	20-21	-0.026992733849532158
1101	22-23	-0.019390049408997356
1101	24-25	-0.0784288756493865
1101	26-27	-0.07753550316471092
1101	28-29	-0.06758100647976306
1101	30-31	-0.009673091972636882
1101	32-33	0.003581815261306076
1101	34-35	-0.05841986661292964
1101	36-37	0.0044280494022075345
1101	38-39	0.034858628893651655
1101	40-41	0.09003226115097362
1101	42-43	0.015695315299701917
1101	44-45	-0.011327596898375702
1101	46-47	0.05841258551311057
1101	48-49	-0.006212468998775478
1101	50-51	0.012345415885860689
1101	52-53	-0.01716814265299149
1101	54-55	0.022771072628003708
1101	56-57	-0.04137679939902483
1101	58-59	-0.05736382417114072
1101	60-61	-0.16378086610687603
1101	62-63	-0.1176011868348894
1101	64-65	-0.08710678492120394
1101	66-67	-0.08015484836927556
1101	68-69	-0.056856611837936555
1101	70-71	-0.05425254297029625
1101	72-73	-0.05827650236148685
1101	74-75	-0.040771205837224045
1101	76-77	-0.05172769009352152
1101	78-79	-0.013159816204392882
1101	80-81	-0.054526086991756983
1101	82-83	-0.011362415465740128
1101	84-85	-0.03078131070509471
1101	86-87	-0.04403733887250638
1101	88-89	-0.0071558441996799615
1101	90-91	-0.05623191069989275
1101	92-93	-0.010648266963308117
1101	94-95	-0.04903472967708211
1101	96-97	0.015724506291284968
1101	98-99	-0.11870882002910577
1101	100	-0.051301940122012724
1102	1	0.10796529101992292
1102	2	0.06520859086054287
1102	3	0.04977294478173633
1102	4	0.025861542174276053
1102	5	-0.006034902225579231
1102	6	0.006234732614075256
1102	7	-0.008580089986722328
1102	8	0.0258923090503842
1102	9	0.02120929101359792
1102	10-11	0.023288472712835073
1102	12-13	-0.011100239308689197
1102	14-15	0.06507867647753329
1102	16-17	0.028457564007844383
1102	18-19	-0.029089296927352848
1102	20-21	-0.03607811801033023
1102	22-23	-0.06601532552369349
1102	24-25	-0.03857053414319722
1102	26-27	-0.06114484895135064
1102	28-29	-0.03335406294185361
1102	30-31	-0.05829706766952114
1102	32-33	-0.066646839831499
1102	34-35	-0.05040154380412787
1102	36-37	-0.05261689286227522
1102	38-39	-0.030650401158041518
1102	40-41	0.04165031148200882
1102	42-43	0.020117867197363637
1102	44-45	0.005718444462040395
1102	46-47	0.05398548482194343
1102	48-49	-0.04836898351647534
1102	50-51	-2.1096628375971704E-4
1102	52-53	-0.02205714730764896
1102	54-55	-0.07703659901127224
1102	56-57	-0.09227888977441978
1102	58-59	-0.09670636438561786
1102	60-61	-0.07047410018945044
1102	62-63	-0.038882057595486685
1102	64-65	-0.12863856209605729
1102	66-67	-0.09750100099721237
1102	68-69	-0.059707177911782594
1102	70-71	-0.043450241039082016
1102	72-73	-0.051257663625499106
1102	74-75	-0.125001072520476
1102	76-77	-0.11356756427020542
1102	78-79	0.0012364606198644879
1102	80-81	-0.015271450952894838
1102	82-83	0.004544279010453067
1102	84-85	-0.022109195344739874
1102	86-87	-0.07179410202624581
1102	88-89	0.010496865218676987
1102	90-91	0.04053424624956392
1102	92-93	-0.005222345215351254
1102	94-95	-0.08993403991717486
1102	96-97	-0.12402416564984264
1102	98-99	-0.1843668020866147
1102	100	0.01740862647520558
1103	1	0.10479198121802824
1103	2	0.08130398030553465
1103	3	0.02262126409938503
1103	4	0.026525446770399697
1103	5	0.009782067149657792
1103	6	0.028751726144420786
1103	7	0.02938523201947163
1103	8	0.03040825437246042
1103	9	0.05301929240832237
1103	10-11	0.016415116647550576
1103	12-13	0.006998090275857294
1103	14-15	0.031655959302334225
1103	16-17	0.02091603913707729
1103	18-19	0.054901695028775066
1103	20-21	0.05083414437316236
1103	22-23	-0.02362234373003247
1103	24-25	-0.044039325247716476
1103	26-27	-0.06066496139002453
1103	28-29	-0.05474906918531275
1103	30-31	-0.09693077989206955
1103	32-33	-0.13706683788439022
1103	34-35	-0.07941953771798893
1103	36-37	0.004308407833363503
1103	38-39	-0.011608697256079381
1103	40-41	-0.015406581074479675
1103	42-43	-0.06844996627069833
1103	44-45	0.01511420337124747
1103	46-47	-0.04209412245618438
1103	48-49	0.014597742398649416
1103	50-51	0.02509641104773408
1103	52-53	0.04760876632150968
1103	54-55	0.011761108143026888
1103	56-57	0.019603795396896828
1103	58-59	0.013705529373375214
1103	60-61	0.04508213574440134
1103	62-63	0.01751661324388465
1103	64-65	0.09558046271993703
1103	66-67	0.04443924312812442
1103	68-69	0.04584510805212716
1103	70-71	0.03640936347584045
1103	72-73	-0.04943210639502382
1103	74-75	-0.10935731204938293
1103	76-77	-0.11290839950679299
1103	78-79	-0.011695762921270614
1103	80-81	-0.044139443102025666
1103	82-83	-0.0275762060282716
1103	84-85	-0.04587889057972916
1103	86-87	-0.014081948097995678
1103	88-89	-0.02111417800768578
1103	90-91	-0.06013905436788036
1103	92-93	-0.08194616497076623
1103	94-95	0.005167767753341934
1103	96-97	-0.06041707849451683
1103	98-99	-0.05560259587312544
1103	100	0.040163808900047115
1104	1	0.10977625009898873
1104	2	0.07243525365223036
1104	3	0.03777620543474569
1104	4	0.02742258702024003
1104	5	0.024232284913594526
1104	6	0.015805052707762002
1104	7	-0.01998744001350161
1104	8	0.018393236890759113
1104	9	0.0048529945347084436
1104	10-11	-0.012470607817668622
1104	12-13	0.0076239638918096375
1104	14-15	0.04328081527465599
1104	16-17	0.01921059469007247
1104	18-19	0.018683159277479433
1104	20-21	-0.023685329990847492
1104	22-23	-0.03468727454045961
1104	24-25	-0.05589835184853342
1104	26-27	-0.04408864321179351
1104	28-29	-0.036699131461027434
1104	30-31	-0.07996128426906779
1104	32-33	-0.05860208438416237
1104	34-35	-0.11629323516900314
1104	36-37	-0.04347724548257759
1104	38-39	-0.03367941303272204
1104	40-41	-0.008831171698112428
1104	42-43	-0.008665782586554371
1104	44-45	-0.05639650681853681
1104	46-47	-0.03172165041323893
1104	48-49	-0.05480261726169999
1104	50-51	-0.07129884913500462
1104	52-53	-0.04878535450785648
1104	54-55	-0.15632833163289916
1104	56-57	-0.09748131778511748
1104	58-59	-0.06590135886222726
1104	60-61	-0.07781602536606869
1104	62-63	-0.0621183231909157
1104	64-65	-0.07101086141243229
1104	66-67	-0.020033011070466955
1104	68-69	0.01808704183237353
1104	70-71	0.014890597387498872
1104	72-73	-0.02689335095494272
1104	74-75	-0.030185455739804468
1104	76-77	-0.10304796627369939
1104	78-79	-0.00487133781217608
1104	80-81	-0.036427227142823426
1104	82-83	-0.06373874591233886
1104	84-85	-0.04946339682151546
1104	86-87	-0.03595860518694849
1104	88-89	0.07234744830264361
1104	90-91	0.053628496365512035
1104	92-93	-0.04799249617247625
1104	94-95	-0.015218615124076962
1104	96-97	-0.052872112908353586
1104	98-99	-0.022846555021544646
1104	100	0.015130003615272614
1105	1	0.17149195647069604
1105	2	0.12482407789305938
1105	3	0.0960322226590975
1105	4	0.07928055854161187
1105	5	0.02318076568641203
1105	6	0.021436598161102438
1105	7	0.04370133719854863
1105	8	0.006019865149397674
1105	9	0.0022952806286440364
1105	10-11	0.050557552135295225
1105	12-13	0.04816619949983192
1105	14-15	0.0873024788614174
1105	16-17	0.06912178944400438
1105	18-19	0.050951510363418606
1105	20-21	0.016455671223759794
1105	22-23	-0.016626731206940804
1105	24-25	-2.457483925226711E-4
1105	26-27	-1.4986647087056326E-4
1105	28-29	0.02122497284604208
1105	30-31	-0.03429164758248504
1105	32-33	-0.006643558544418227
1105	34-35	0.01973692940443783
1105	36-37	0.033115390656384136
1105	38-39	0.040397764920690804
1105	40-41	0.03570617901215911
1105	42-43	-0.006938399274567075
1105	44-45	0.034136634305696134
1105	46-47	0.07855535021236193
1105	48-49	0.09452860351305503
1105	50-51	0.014209410989195703
1105	52-53	0.02859647747534666
1105	54-55	0.0577783418206792
1105	56-57	0.029405909446161616
1105	58-59	0.04766889113502515
1105	60-61	-0.006885956325319853
1105	62-63	-0.01728735939462922
1105	64-65	0.024704145343619643
1105	66-67	0.008319505157778906
1105	68-69	-0.004985816257480735
1105	70-71	-0.09435381866768466
1105	72-73	-0.07137655390801712
1105	74-75	-0.039791427075833496
1105	76-77	-0.02582387435905531
1105	78-79	0.02178521477476636
1105	80-81	0.00923436779764586
1105	82-83	0.03242365810901049
1105	84-85	0.08245745173184105
1105	86-87	0.06683907604785588
1105	88-89	0.06108035276884749
1105	90-91	0.050320008291109275
1105	92-93	0.05529473244946104
1105	94-95	0.015012323029594654
1105	96-97	0.017374984443691233
1105	98-99	0.05213792684534013
1105	100	0.04380875480065782
1106	1	0.11133344440620618
1106	2	0.0776463131808427
1106	3	0.040656434439704014
1106	4	0.033183939348766955
1106	5	0.034423652896407475
1106	6	0.05696090314771851
1106	7	0.04549817937705569
1106	8	0.03476689440884684
1106	9	0.019018174438876656
1106	10-11	0.028475828639919598
1106	12-13	0.008048824288671597
1106	14-15	0.0858601762641058
1106	16-17	0.06211115105073617
1106	18-19	0.03304665011415864
1106	20-21	0.010674880215198357
1106	22-23	0.00462641456159929
1106	24-25	0.05533714829392267
1106	26-27	-0.021760165252288743
1106	28-29	0.02584307447131806
1106	30-31	-0.034301501898291065
1106	32-33	-0.02274263340398619
1106	34-35	0.014278141951386658
1106	36-37	0.037452032881951425
1106	38-39	0.052279648166276615
1106	40-41	0.02086919984571267
1106	42-43	0.068671296355582
1106	44-45	0.046349032742107
1106	46-47	-0.02877164768303686
1106	48-49	-0.012492311181325988
1106	50-51	-0.03813218304205179
1106	52-53	-0.014178877643274745
1106	54-55	0.0015467865943392667
1106	56-57	5.441769097487281E-4
1106	58-59	-0.03528107095102939
1106	60-61	0.025754894335669576
1106	62-63	-0.015323928928431485
1106	64-65	-0.0163878720515811
1106	66-67	0.04248355491617417
1106	68-69	0.0643973903675672
1106	70-71	-0.01233145699529814
1106	72-73	-0.044435022321003714
1106	74-75	-0.06225885952114396
1106	76-77	-0.053941066582034125
1106	78-79	0.02297447005079789
1106	80-81	0.07436724737210199
1106	82-83	0.05799227490881265
1106	84-85	0.07982232136931344
1106	86-87	0.037142651793040216
1106	88-89	0.04428011097126472
1106	90-91	0.09131808600421465
1106	92-93	0.05479513542144332
1106	94-95	0.09041102600982498
1106	96-97	0.08229701522684962
1106	98-99	0.14710387239188094
1106	100	0.1410681185225897
1107	1	0.10072475841718642
1107	2	0.05602583399003436
1107	3	0.027873491073705736
1107	4	0.03036564823315757
1107	5	0.021443361276453743
1107	6	0.05159744748978312
1107	7	0.021116169032019627
1107	8	0.021016408477507298
1107	9	-0.052393251190515855
1107	10-11	-0.019137491221208336
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2315	98-99	0.020292337371287772
2315	100	-0.12291035539559303
2316	1	0.0591243308406888
2316	2	-0.05552970523178402
2316	3	-0.021090292954070833
2316	4	-0.17895558543394685
2316	5	-0.12878066407746758
2316	6	-0.05955384683964127
2316	7	-0.03630314132674073
2316	8	0.008561755991451037
2316	9	-0.03233954882532686
2316	10-11	-0.05196429340863773
2316	12-13	-0.023124996278376386
2316	14-15	-0.024715749161686063
2316	16-17	-0.01628141136404082
2316	18-19	0.018977687591750225
2316	20-21	0.019706388786531193
2316	22-23	-0.032818336266210224
2316	24-25	-0.020413771925682056
2316	26-27	-0.03363100619709769
2316	28-29	-0.07680654433278988
2316	30-31	-0.3493723108630604
2316	32-33	-0.16099352774791953
2316	34-35	-0.23281967652886948
2316	36-37	-0.45766868831022123
2316	38-39	-0.3307167700418958
2316	40-41	-0.2251981686429403
2316	42-43	-0.38388232483396934
2316	44-45	-0.22167077776881428
2316	46-47	-0.4045161829353887
2316	48-49	-0.33575798180229555
2316	50-51	-0.3434766122340278
2316	52-53	-0.3542933636021104
2316	54-55	-0.19787190997900694
2316	56-57	-0.9972613126072432
2316	58-59	-0.7587748661474976
2316	60-61	-0.41916386086621316
2316	62-63	-0.21584400935142156
2316	64-65	-0.13903936678482864
2316	66-67	-0.09838736105659507
2316	68-69	-0.16772420682554667
2316	70-71	-0.2119220830721673
2316	72-73	-0.12820105448844998
2316	74-75	-0.1301339824421177
2316	76-77	-0.1188670752539096
2316	78-79	-0.13814718836992768
2316	80-81	-0.17893601865364417
2316	82-83	-0.35429053442709346
2316	84-85	-0.13868209113526575
2316	86-87	-0.20694763090782686
2316	88-89	-0.2734000933440015
2316	90-91	-0.16754410363198247
2316	92-93	-0.1444136806542815
2316	94-95	-0.16981369631655951
2316	96-97	-0.35352472568783355
2316	98-99	-0.1563453531571355
2316	100	-0.3240772514952326
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	9.0
13	57.0
14	230.0
15	703.0
16	1653.0
17	3502.0
18	6579.0
19	10185.0
20	15784.0
21	24346.0
22	32315.0
23	33467.0
24	33001.0
25	36618.0
26	44776.0
27	56476.0
28	71372.0
29	89010.0
30	109580.0
31	137517.0
32	174413.0
33	232003.0
34	353264.0
35	556680.0
36	1012437.0
37	2101523.0
38	2185714.0
39	398447.0
40	30.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.1793565425796	20.730004704928216	13.727719825423767	23.362918927068414
2	15.631074235289836	20.73215948247337	39.491243517739925	24.145522764496867
3	18.690289714960265	26.612282111569495	30.060085047636132	24.63734312583411
4	13.038603169242	17.680313158138073	39.834521214143095	29.446562458476837
5	13.591172528120083	38.61917928859591	34.30836576505404	13.481282418229974
6	29.525769740621616	38.00871622437155	18.62156636136225	13.843947673644585
7	26.8944492721153	32.67907673061208	22.71336974159802	17.7131042556746
8	26.792109322479373	34.02948248623452	20.15191275357698	19.026495437709126
9	26.453775627000436	15.61268446350593	21.203670918633634	36.729868990860005
10-11	25.638846796043403	25.75384757242815	27.589346732132498	21.017958899395946
12-13	26.301946711903756	23.28610962863426	27.56000081329378	22.851942846168203
14-15	23.868425421741453	24.685133042394693	25.239177411530196	26.207264124333662
16-17	23.040703888045126	27.419535833026025	26.08818091134931	23.45157936757954
18-19	22.43707607180916	27.381773129953352	27.929521910954918	22.251628887282575
20-21	24.099518796240766	26.421015797475082	27.354563004901657	22.1249024013825
22-23	24.226849050096735	25.967014177353352	27.05915331130618	22.746983461243737
24-25	23.71323538934213	26.219143420897908	27.40188290338439	22.66573828637558
26-27	23.460275485361688	26.722528181085224	26.685948669147475	23.13124766440561
28-29	22.797744153719275	26.742127058271926	27.386105958202016	23.07402282980678
30-31	23.683325261662333	26.235462655036468	27.364771342479155	22.716440740822044
32-33	23.729050433998257	26.581673654577553	26.562912148421713	23.126363763002477
34-35	23.742117429831396	26.735603105986506	27.119499556524477	22.402779907657617
36-37	23.603022913409056	26.333113038038334	26.68210352756341	23.381760520989197
38-39	23.618790586068776	26.37289735078001	27.046637506554617	22.961674556596602
40-41	23.634555135014327	25.57384578530197	27.670261397210183	23.12133768247352
42-43	23.388917482579792	25.702739960570923	27.239319867906648	23.669022688942633
44-45	23.6183592600375	26.17195318461862	27.06712948048706	23.142558074856822
46-47	23.482025353276647	26.054490784879984	26.460551953339685	24.00293190850368
48-49	24.033416909498644	25.892975794170013	26.937822493343223	23.13578480298812
50-51	23.78074706119813	25.923990235486205	26.67028991654265	23.624972786773018
52-53	23.362564351627	26.116729804112026	26.631659381576817	23.889046462684153
54-55	23.332718368083835	25.998375792377544	26.60967801615362	24.059227823385
56-57	24.04318929756427	25.829918004258527	26.210126641457563	23.91676605671964
58-59	22.981810279588867	26.249431548978286	27.081275399796787	23.687482771636056
60-61	23.435404636385876	26.402797273798996	26.528631598823104	23.63316649099203
62-63	23.738410215011328	25.891223683538282	26.297964940372264	24.072401161078126
64-65	22.878641743277438	25.904246656727096	26.810016625305757	24.40709497468971
66-67	23.536476011089977	25.970334004699563	26.512574907065506	23.980615077144954
68-69	23.037631947260337	25.846517105177448	26.817662669067392	24.298188278494827
70-71	22.962324639098973	25.686548721079788	26.659214854308587	24.691911785512648
72-73	23.18384108023829	26.036441322821275	26.563950195309022	24.21576740163141
74-75	23.320407483838263	26.231284786613397	26.489868692787798	23.95843903676054
76-77	22.980636657059595	26.2247658907187	26.028784439700914	24.765813012520795
78-79	23.01444159621071	26.116934172344553	26.235485507151125	24.633138724293616
80-81	22.892612688799453	26.67631701892827	26.343577362488148	24.087492929784133
82-83	23.44677806304979	25.986557320788563	26.95684550308061	23.609819113081034
84-85	23.420454494681746	26.113969520037035	26.25381164659053	24.21176433869069
86-87	22.375875929741458	26.658478997307082	26.692909429120515	24.27273564383094
88-89	23.0209633804568	25.680043928947	26.59908749009268	24.699905200503526
90-91	22.98230630012271	25.94361327043679	26.361968096300348	24.712112333140155
92-93	23.004418035177476	26.04222840752327	26.542608383932098	24.41074517336715
94-95	22.326962511396047	25.949032863292814	26.995568759218262	24.72843586609288
96-97	22.51067259545827	26.00505197902203	26.654884457138245	24.829390968381453
98-99	22.155577842537806	26.187755277538116	26.574292967504494	25.082373912419587
100	22.585372234338493	25.692768743815954	26.174282650476332	25.547576371369217
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	85.0
1	57.5
2	61.5
3	91.5
4	66.5
5	72.0
6	148.0
7	286.5
8	491.5
9	662.5
10	663.5
11	648.0
12	754.0
13	944.0
14	1189.0
15	1464.5
16	1779.5
17	2128.0
18	2592.5
19	3047.0
20	3487.5
21	4014.5
22	4649.5
23	5325.0
24	5990.0
25	6827.0
26	8163.0
27	9994.0
28	12259.5
29	15924.5
30	21734.0
31	29412.0
32	41684.5
33	59290.0
34	78958.5
35	100646.0
36	133227.5
37	177391.0
38	212495.0
39	238521.0
40	250649.5
41	253589.5
42	263583.0
43	282692.0
44	307344.5
45	345316.0
46	380698.5
47	404777.5
48	421624.5
49	412900.0
50	390339.5
51	375532.0
52	360286.0
53	342192.5
54	326855.0
55	289082.5
56	250371.5
57	204850.0
58	158732.0
59	139414.0
60	110528.0
61	77204.0
62	56802.5
63	44590.5
64	31324.5
65	16715.0
66	9977.0
67	7033.5
68	5324.0
69	3954.0
70	2926.5
71	2181.0
72	1638.5
73	1168.5
74	834.0
75	566.0
76	355.5
77	222.0
78	138.5
79	75.5
80	51.0
81	26.5
82	14.0
83	11.0
84	6.5
85	3.5
86	2.5
87	2.0
88	1.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3578490310154821
2	5.309717093093068E-4
3	0.0017742225408628057
4	0.0012691518905441968
5	0.00442908108740934
6	0.0
7	2.5901058990697897E-5
8	2.978621783930258E-4
9	0.0031858302558558406
10-11	6.475264747674474E-5
12-13	6.475264747674474E-5
14-15	2.3310953091628105E-4
16-17	1.23030030205815E-4
18-19	0.0011202208013476839
20-21	1.9425794243023423E-4
22-23	1.4245582444883843E-4
24-25	0.0
26-27	0.0020267578660221105
28-29	0.011195732748729165
30-31	0.0031405034026221195
32-33	0.014200255591650121
34-35	0.011137455366000094
36-37	0.012879301583124529
38-39	0.012762746817666386
40-41	0.014057799767201281
42-43	0.006293957334739589
44-45	0.0010943197423569858
46-47	0.00571765877219656
48-49	0.012821024200395457
50-51	0.003405989257276773
52-53	0.005724134036944235
54-55	0.005031280708943066
56-57	1.3598055970116395E-4
58-59	6.540017395151217E-4
60-61	4.0146641435581735E-4
62-63	9.777649768988456E-4
64-65	3.820406201127939E-4
66-67	0.0016058656574232694
68-69	0.0018260246588442015
70-71	0.0010425176243755903
72-73	0.0027260864587709535
74-75	0.007776792961957043
76-77	0.0038592577896139862
78-79	0.001845450453087225
80-81	5.115459150662833E-4
82-83	2.201590014209321E-4
84-85	0.0016511925106569905
86-87	0.004208922085988408
88-89	0.001709469893386061
90-91	2.201590014209321E-4
92-93	0.0020720847192558316
94-95	0.003438365581015146
96-97	0.0021174115724895528
98-99	0.002512402722097696
100	0.0027455122530139764
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7721692.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.871292844549934
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.21169764159471	39.930300176881296
2	16.22438319546905	18.4540329586343
3	6.194882943736832	10.569330060928948
4	2.867679073845368	6.523544655713906
5	1.5488158164775778	4.404157893058351
6	0.8980335729773828	3.0643398187820554
7	0.534396351005936	2.1274267961182236
8	0.37047603251951755	1.6855560749843588
9	0.24564861764784082	1.257331902399831
>10	0.8548242885294698	7.627696951201092
>50	0.028943391012883225	1.1266370068222593
>100	0.018396282690863925	2.0245794210714174
>500	0.001253185997470321	0.4738469019311935
>1k	5.126458459171896E-4	0.446182308969696
>5k	5.6960649153871353E-5	0.2850370725032161
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9544	0.12359985350361034	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8054	0.10430356455554042	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.2950529495348947E-5	0.0
4	0.0	0.0	0.0	3.885158848604684E-5	0.0
5	0.0	0.0	0.0	3.885158848604684E-5	0.0
6	0.0	0.0	0.0	5.180211798139579E-5	0.0
7	0.0	0.0	0.0	5.180211798139579E-5	0.0
8	0.0	0.0	0.0	6.475264747674474E-5	0.0
9	2.5901058990697894E-5	0.0	0.0	1.942579424302342E-4	1.2950529495348947E-5
10-11	2.5901058990697894E-5	0.0	0.0	3.3023850213139815E-4	1.2950529495348947E-5
12-13	2.5901058990697894E-5	0.0	0.0	3.4318903162674705E-4	1.2950529495348947E-5
14-15	2.5901058990697894E-5	0.0	0.0	4.403180028418642E-4	1.2950529495348947E-5
16-17	2.5901058990697894E-5	0.0	0.0	4.8564485607558556E-4	1.2950529495348947E-5
18-19	5.180211798139579E-5	0.0	0.0	5.180211798139579E-4	1.2950529495348947E-5
20-21	1.424558244488384E-4	0.0	0.0	6.15150151029075E-4	1.2950529495348947E-5
22-23	1.8130741293488525E-4	0.0	0.0	8.35309152450007E-4	1.2950529495348947E-5
24-25	1.8778267768255973E-4	0.0	0.0	0.001256201361048848	1.2950529495348947E-5
26-27	1.942579424302342E-4	0.0	0.0	0.0018519257178348995	1.942579424302342E-5
28-29	1.942579424302342E-4	0.0	0.0	0.0035549203464732854	2.5901058990697894E-5
30-31	2.0073320717790868E-4	0.0	0.0	0.0095380649733245	2.5901058990697894E-5
32-33	2.0720847192558315E-4	0.0	0.0	0.025933435314436266	3.885158848604684E-5
34-35	2.0720847192558315E-4	0.0	0.0	0.05388067796539929	3.885158848604684E-5
36-37	2.0720847192558315E-4	0.0	0.0	0.10001046402783224	3.885158848604684E-5
38-39	2.0720847192558315E-4	0.0	0.0	0.17306440091109565	3.885158848604684E-5
40-41	2.0720847192558315E-4	0.0	0.0	0.2571845652481347	3.885158848604684E-5
42-43	2.0720847192558315E-4	0.0	0.0	0.34451646090002036	3.885158848604684E-5
44-45	2.0720847192558315E-4	0.0	0.0	0.4559039650895167	3.885158848604684E-5
46-47	2.0720847192558315E-4	0.0	0.0	0.5877144542931783	3.885158848604684E-5
48-49	2.0720847192558315E-4	0.0	0.0	0.7374938031716365	3.885158848604684E-5
50-51	2.0720847192558315E-4	0.0	0.0	0.9011884959928471	3.885158848604684E-5
52-53	2.0720847192558315E-4	0.0	0.0	1.0664631534124904	4.532685323372131E-5
54-55	2.201590014209321E-4	0.0	0.0	1.2447271919159686	5.180211798139579E-5
56-57	2.3310953091628105E-4	0.0	0.0	1.4507830667164658	5.180211798139579E-5
58-59	2.3310953091628105E-4	0.0	0.0	1.6944019523182225	5.180211798139579E-5
60-61	2.4606006041163E-4	0.0	0.0	1.9506346536484491	5.180211798139579E-5
62-63	2.5901058990697895E-4	0.0	0.0	2.2159327256254198	5.180211798139579E-5
64-65	2.7843638415000235E-4	0.0	0.0	2.4926337388230455	6.475264747674474E-5
66-67	2.978621783930258E-4	0.0	0.0	2.7958380106329024	6.475264747674474E-5
68-69	2.978621783930258E-4	0.0	0.0	3.132182687421358	6.475264747674474E-5
70-71	2.978621783930258E-4	0.0	0.0	3.508945966764797	6.475264747674474E-5
72-73	3.0433744314070025E-4	0.0	0.0	3.8956423022311686	7.770317697209367E-5
74-75	3.108127078883747E-4	0.0	0.0	4.2819954486659135	7.770317697209367E-5
76-77	3.367137668790726E-4	0.0	0.0	4.708352780711792	7.770317697209367E-5
78-79	3.4966429637442155E-4	0.0	0.0	5.1722407472351914	7.770317697209367E-5
80-81	3.4966429637442155E-4	0.0	0.0	5.665552316772024	9.065370646744263E-5
82-83	3.4966429637442155E-4	0.0	0.0	6.178289680551879	1.0360423596279158E-4
84-85	3.4966429637442155E-4	0.0	0.0	6.695061134269536	1.0360423596279158E-4
86-87	3.626148258697705E-4	0.0	0.0	7.2362054844974395	1.0360423596279158E-4
88	3.626148258697705E-4	0.0	0.0	7.656547295592728	1.0360423596279158E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12100	0.0	43.651596	1
GTATCAA	21425	0.0	39.796516	1
CAACGCA	23045	0.0	35.07661	5
ATCAACG	24215	0.0	33.44068	3
TATCAAC	26480	0.0	31.89284	2
AACGCAG	27760	0.0	30.404362	6
ACGCAGA	28670	0.0	28.242725	7
TCAACGC	29495	0.0	27.900146	4
CGCAGAG	29015	0.0	27.825926	8
GTACTAG	6385	0.0	26.072416	1
GCAGAGT	32060	0.0	24.758139	9
CGTTAAC	2070	0.0	24.377037	1
GTGGTAT	4285	0.0	23.552143	1
GGGCACC	7000	0.0	23.43098	94
TAGGCAT	8340	0.0	23.329218	5
CTGTGCG	8055	0.0	22.111872	9
GAGTACT	11605	0.0	21.989052	12-13
GTGATCG	4245	0.0	21.919798	8
GTACATG	34980	0.0	21.678722	1
TACATGG	35410	0.0	21.089247	2
>>END_MODULE