FastQCFastQC Report
Thu 26 May 2016
ERR580153_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580153_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3647889
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83690.2294203579111097No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65580.17977520697587016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62800.049.8020021
GTATCAA116350.042.4835851
CAACGCA133900.035.437225
ATCAACG137150.034.7384453
TATCAAC142800.034.2861942
AACGCAG153950.031.6594836
TCAACGC151100.031.5922434
GTGGTAT22900.030.1902481
ACGCAGA165800.028.5742477
CGCAGAG170350.027.756628
GTACTAG21750.027.0293481
GCAGAGT185050.025.3742439
GTACATG177150.023.6018141
TATTCGC2602.7284841E-1123.5010199
TACATGG178050.023.03412
TAGGCAT29050.022.5018235
GGGCTAT32500.022.4157416
GCTATTG32550.022.2372178
GAGTACT80150.022.1948512-13
ACATGGG177950.022.016273
Produced by FastQC (version 0.11.5)