FastQCFastQC Report
Thu 26 May 2016
ERR580153_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580153_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3647889
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70220.19249489225138156No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56880.15592579708428628No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63900.048.336041
GTATCAA113450.040.650981
CAACGCA132450.033.7114145
ATCAACG136000.032.8318983
TATCAAC142350.032.127182
AACGCAG148250.031.0362686
TCAACGC149900.029.9438134
GTGGTAT21600.028.0764681
ACGCAGA162500.027.5915787
CGCAGAG167550.026.647768
GTACTAG24050.025.9981881
GCAGAGT183550.024.1456459
CGTTAAC8200.023.5058821
GGGCTAT33050.022.6103446
GTACATG172100.022.563461
GCTATTG31200.021.9927628
TACATGG174500.021.9522692
TAGGCAT31250.021.8083745
CTAGGCA32650.021.7369754
AGGGCTA33100.021.7254495
Produced by FastQC (version 0.11.5)