FastQCFastQC Report
Thu 26 May 2016
ERR580152_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580152_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8012269
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257200.32100769457440836No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199840.24941748710633654No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123460.15408868573933301No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA148250.050.185561
GTATCAA282400.043.198861
CAACGCA320850.036.5697755
ATCAACG329050.035.655773
TATCAAC355550.034.0904272
GTACTAG73000.032.9203951
AACGCAG366400.032.8786776
ACGCAGA377700.030.8011057
TCAACGC387500.030.5580254
CGCAGAG382600.030.3327588
GCATCGT73200.030.23228
TAGGCAT89500.030.043655
GGGCACC73550.028.5689194
GCAGAGT407500.027.9983569
GTGGTAT41850.027.9814931
CTAGGCA98600.027.7469274
GTACATG400000.027.5355591
TACATGG408150.026.7020052
ACATGGG398250.026.5448843
CTGTGCG92650.026.4243189
Produced by FastQC (version 0.11.5)