FastQCFastQC Report
Thu 26 May 2016
ERR580152_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580152_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8012269
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229100.2859364806648404No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189090.23600056363559438No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116650.14558922073135588No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA153950.051.54961
GTATCAA277550.040.2812771
GTGGTAT39600.034.6673161
CAACGCA322100.033.197395
ATCAACG328350.032.5798073
GTACTAG73050.031.600491
TATCAAC357500.030.99812
GGGCACC74350.030.08742594
AACGCAG372100.029.7452346
TAGGCAT88800.028.7938165
TCAACGC385800.027.9232314
ACGCAGA385800.027.5434977
CGCAGAG390300.027.1898088
AGGGCTA94550.026.5953395
TATTCTC43150.026.5780015
GCTATTG93800.026.5554588
TACCCCG6400.026.438445
CTAGGCA100200.025.8462284
ATTTAGG99000.025.8342741
CTATTGA97250.025.8054089
Produced by FastQC (version 0.11.5)