FastQCFastQC Report
Thu 26 May 2016
ERR580151_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580151_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6275525
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218500.3481780408810418No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176040.2805183630054856No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109780.17493357129483192No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108300.050.2017481
GTATCAA192900.039.790321
CAACGCA224150.033.0854765
ATCAACG225200.032.893653
GTACTAG43700.032.6099931
GTGGTAT38950.032.1191251
TATCAAC243100.031.381182
AACGCAG250100.030.541736
GTACATG262350.029.5975321
TCAACGC252750.029.5677834
TACATGG267150.028.591322
GCATCGT46750.028.4538788
ACATGGG261800.028.2052463
TAGGCAT56050.027.771345
ACGCAGA267850.027.6578567
CGCAGAG274250.026.9599298
CATGGGG201300.026.2374734
CTGTGCG58050.025.9911589
TAGTACT60600.025.4558284
ATGGGGG158800.025.3494015
Produced by FastQC (version 0.11.5)