FastQCFastQC Report
Thu 26 May 2016
ERR580151_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580151_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6275525
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189700.30228546615621804No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158390.25239322606475156No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103460.1648627007302178No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103100.048.5156361
GTATCAA187250.040.671661
CAACGCA220900.033.2133335
ATCAACG221800.033.1000183
TATCAAC240500.031.5240422
GTACTAG43950.030.3779321
AACGCAG249650.030.102426
TCAACGC252950.029.1722744
GTACATG251100.028.7569771
TACCTGG33300.027.9475362
TACATGG258400.027.5031132
ACATGGG252000.027.062983
ACGCAGA272150.026.9056597
GTGGTAT38700.026.4816041
TAGTACT61300.026.3756564
CGCAGAG278300.026.2941998
CTGTGCG59400.026.2685559
TAGGCAT55650.025.5062775
GGGCACC57050.025.29490394
CATGGGG192700.024.6101784
Produced by FastQC (version 0.11.5)