FastQCFastQC Report
Thu 26 May 2016
ERR580150_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580150_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8087636
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199240.24635134419996155No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154090.19052538961941412No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC112470.13906412207473234No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA100470.12422665906329118No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93970.11618969993209388No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC100950.043.20546394
GGTATCA151400.039.0800821
GTATCAA254600.037.2235151
CAACGCA254500.034.7946245
GTGGTAT28250.034.6294481
TATTCTC38450.033.389965
ATCAACG266600.033.1842543
GTACTAG112750.032.6622351
TATCAAC307600.030.6880872
CTGTGCG156200.030.6024179
ACCTGGG19550.030.5534573
TAGGCAT132200.030.4147555
TAGTACT158700.030.2280754
AGGGCTA99950.030.2066025
GCATCGT115950.030.1573938
TACCCCG5800.030.0001455
ACTGTGC161050.029.9710088
AACGCAG315500.029.4527456
ACGCAGA309950.028.5693057
TACTGTG177650.028.520957
Produced by FastQC (version 0.11.5)