FastQCFastQC Report
Thu 26 May 2016
ERR580150_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580150_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8087636
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT342940.4240299637619695No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162760.20124545664518037No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134550.1663650540157841No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85960.1062856933719569No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC99450.043.0122194
GGTATCA152400.038.2180371
GTATCAA250750.036.2762221
GTGGTAT26350.035.145331
GTACTAG108050.034.805451
CAACGCA258800.033.0709885
ATCAACG270750.031.6809793
TATTCTC41600.031.5218965
TAGGCAT130950.031.3694885
CTGTGCG156750.029.6533729
TAGTACT158000.029.5985324
ACTGTGC161900.029.2614888
TATCAAC313000.029.1171172
AGGGCTA102000.029.0757355
TTAAGGT17950.028.5408044
CTAGGCA150900.028.2502464
CTAACGC53100.028.1473033
TACCCCG4850.028.1033295
TACTGTG176550.027.8715827
GTACTGT168250.027.7381116
Produced by FastQC (version 0.11.5)