FastQCFastQC Report
Thu 26 May 2016
ERR580149_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580149_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9792293
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247330.2525761841480846No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203610.20792882729305587No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112020.11439608680009883No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA149400.050.9256941
GTATCAA264500.040.3561021
CAACGCA312700.032.8673555
ATCAACG317150.032.453643
TATCAAC333400.031.931542
AACGCAG345700.030.3940476
TCAACGC342000.030.2867744
GTACTAG48800.028.9074941
GTGGTAT56500.028.296941
ACGCAGA379450.027.0464177
CGCAGAG392400.026.0931558
GTACATG365950.025.1465321
TACATGG368900.024.4483172
ACATGGG370650.023.494133
GCAGAGT432350.023.1972859
GCATCGT55800.022.7367258
CATGGGG294400.021.4328214
TAGGCAT68550.021.1244645
GAGTACT226650.020.92977312-13
TGGTATC72550.020.4813542
Produced by FastQC (version 0.11.5)