FastQCFastQC Report
Thu 26 May 2016
ERR580149_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580149_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9792293
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218480.22311423892238516No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194060.19817625963602192No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107680.10996402987533155No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA145250.050.5256351
GTATCAA259150.041.659391
CAACGCA316400.033.123485
ATCAACG320050.032.6876563
TATCAAC332750.032.0891272
GTACTAG48350.031.8984531
AACGCAG346350.030.7868146
TCAACGC345350.030.4966564
GTGGTAT54100.030.4261131
ACGCAGA387800.026.926617
CGCAGAG400500.025.8734138
GTACATG374050.023.7684041
TAGGCAT69650.023.4814955
GCAGAGT443600.023.0736459
TACATGG376200.022.8612482
TGGTATC69450.022.8047472
ACATGGG375550.021.862593
CTAGGCA76300.021.804624
TAGTACT76250.021.5107444
CTGTGCG77800.021.1416239
Produced by FastQC (version 0.11.5)