FastQCFastQC Report
Thu 26 May 2016
ERR580148_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580148_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7955193
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195700.24600283110667462No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160440.2016795821295599No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84720.10649647343565392No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA120600.048.19981
GTATCAA222900.041.5441321
CAACGCA248800.035.632535
ATCAACG253200.035.105043
TATCAAC275050.033.3333852
AACGCAG280900.032.263526
TCAACGC291950.030.6556874
ACGCAGA297750.029.6181147
GTGGTAT42350.029.5394421
CGCAGAG302100.029.0983268
GTACTAG53950.027.372361
GCATCGT47450.026.239448
GCAGAGT336750.025.591889
CTGTGCG67650.024.2419839
TACCCCG7450.023.9763265
GTACATG286250.023.9215831
GGGCACC60450.023.40532194
TACATGG292300.023.1144962
ACATGGG284800.022.7761753
GAGTACT183000.021.96951112-13
Produced by FastQC (version 0.11.5)