FastQCFastQC Report
Thu 26 May 2016
ERR580148_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580148_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7955193
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173410.2179833977629455No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149050.18736189052861446No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81510.10246137334443048No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA124600.050.1851541
GTATCAA221150.039.4365231
CAACGCA249050.033.6872065
ATCAACG252050.033.2864573
TATCAAC275150.031.457822
AACGCAG281550.030.5983776
TCAACGC291600.028.9974424
ACGCAGA305550.027.287277
CGCAGAG308100.026.9541368
TAGGCAT68600.024.1859915
GCAGAGT342950.023.8437429
GTGGTAT45400.023.5077821
GGGCACC57650.023.15244394
GTACATG288100.023.14061
GCTATTG66900.022.6209228
TACATGG291700.022.581152
GCATCGT59350.022.5686048
ACATGGG283550.022.3116383
CTGTGCG68300.021.9495689
GAGTACT189550.021.80573712-13
Produced by FastQC (version 0.11.5)