FastQCFastQC Report
Thu 26 May 2016
ERR580147_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580147_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7944822
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196570.2474190107720475No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158670.19971498417459826No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95130.1197383654410382No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119200.050.4168171
GTATCAA213400.041.4270741
CAACGCA244000.034.889835
ATCAACG249750.034.123133
TATCAAC268500.032.584882
AACGCAG272750.031.6779776
TCAACGC277700.030.8252814
GTGGTAT39950.029.1914521
ACGCAGA293000.028.86387
GTACTAG49450.028.1479151
CGCAGAG299850.028.1112848
GTACATG293500.027.0609071
TACATGG299550.026.2253442
ACATGGG298100.025.7029063
GCAGAGT331750.025.028329
TAGTACT67050.024.4680674
CTGTGCG66050.023.9041089
CATGGGG247100.023.037984
CTAGTAC71300.022.8109383
TAGGCAT66100.022.686085
Produced by FastQC (version 0.11.5)