FastQCFastQC Report
Thu 26 May 2016
ERR580147_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580147_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7944822
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171640.2160400824587385No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150900.18993502938140086No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90240.11358341319667074No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116100.049.5684661
GTATCAA206900.040.9042361
CAACGCA243500.033.471085
ATCAACG246250.033.0792433
TATCAAC265550.031.6583612
AACGCAG272700.030.4535686
TCAACGC275750.029.8809364
GTGGTAT40900.029.5437831
ACGCAGA294600.027.5994327
GTACTAG51600.027.5177881
CGCAGAG304250.026.615928
GTACATG286150.024.6135561
TACATGG289300.024.037372
GCAGAGT336200.023.5951799
ACATGGG292450.023.0639423
CTGTGCG65700.022.7462739
TAGTACT65750.022.6616384
TAGGCAT68150.022.6218225
CTAGTAC70200.022.0288623
CTAGGCA75550.021.650734
Produced by FastQC (version 0.11.5)