FastQCFastQC Report
Thu 26 May 2016
ERR580146_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580146_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6093761
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165790.27206514991316527No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136230.22355651952874422No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82700.13571257553422264No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93600.047.231151
GTATCAA163300.041.4429971
CAACGCA185950.034.696985
ATCAACG190550.033.9378663
TATCAAC204650.032.7257042
AACGCAG211550.031.1947086
TCAACGC212050.030.6957244
GTGGTAT32550.030.0530741
GTACTAG36700.029.2176511
ACGCAGA226650.028.5143267
GTACATG220250.027.7803631
CGCAGAG236850.027.3244698
TACATGG221700.027.2813642
ACATGGG221450.026.1653083
GCAGAGT260650.024.4882749
TACCTGG35700.024.1086942
TAGGCAT47100.024.0618955
CTAACGC17600.024.0503083
TCTAACG17550.023.8508422
CATGGGG180800.023.8274274
Produced by FastQC (version 0.11.5)