FastQCFastQC Report
Thu 26 May 2016
ERR580146_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580146_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6093761
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146020.23962213155389586No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126190.2070806518339003No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77170.1266377201206283No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89850.046.4609951
GTATCAA155700.038.677121
CAACGCA182700.031.823125
ATCAACG187650.031.009733
TATCAAC198950.029.8154452
AACGCAG206400.028.9413626
GTACATG216300.028.6017741
TCAACGC207050.028.2394854
GTACTAG34200.028.0412671
TACATGG220650.027.5009782
GTGGTAT33650.027.3819641
ACATGGG220250.026.7826583
ACGCAGA224850.025.897727
CATGGGG178550.025.1394124
CGCAGAG233500.024.8779568
CTAACGC15300.023.962333
TAGTACT53550.023.7860014
CTGTGCG54500.023.714799
ATGGGGG132600.023.6070825
CTAGTAC55800.022.9961073
Produced by FastQC (version 0.11.5)