FastQCFastQC Report
Thu 26 May 2016
ERR580145_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580145_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8433882
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229570.2721996821866846No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188050.22296968347434784No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109420.12973859487244427No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA128050.049.3927081
GTATCAA228800.041.0021861
CAACGCA266650.034.0054365
ATCAACG270700.033.5633433
TATCAAC291300.032.003252
AACGCAG297000.031.0834526
GTACTAG52500.030.9015271
TCAACGC301550.030.3025474
ACGCAGA320050.028.2439777
GTACATG314850.027.9291741
CGCAGAG328150.027.3893158
TACATGG323350.026.7956522
ACATGGG322850.026.1327613
GTGGTAT49050.025.9806771
GCATCGT54650.025.9630328
CTGTGCG67700.025.4028579
TAGGCAT67550.024.6340375
GCAGAGT361400.024.365299
TAGTACT71450.024.2753984
CATGGGG250200.024.1599434
Produced by FastQC (version 0.11.5)