FastQCFastQC Report
Thu 26 May 2016
ERR580145_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580145_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8433882
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202900.24057723359183825No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175500.20808922866124993No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103260.12243472223111493No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130600.051.1578981
GTATCAA231700.040.2603381
CAACGCA268400.033.289475
ATCAACG272900.032.775573
TATCAAC293800.031.3356112
AACGCAG299900.030.5280576
GTACTAG52600.029.8553771
GTGGTAT45400.029.4119821
TCAACGC305900.029.4084634
ACGCAGA325450.027.4237827
CTGTGCG68750.026.7954719
CGCAGAG333900.026.6309268
GTACATG309250.025.8616621
TAGTACT74500.025.4879534
TAGGCAT64800.025.3863535
TACATGG314700.024.9216382
CTAACGC24850.024.3993553
ACATGGG310350.024.3831143
TAACGCC24550.023.9314164
GCAGAGT369050.023.8506729
Produced by FastQC (version 0.11.5)