FastQCFastQC Report
Thu 26 May 2016
ERR580144_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580144_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7109684
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183930.2587034810548542No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147710.20775888211065358No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86200.12124308197101305No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108550.053.4623761
GTATCAA200650.041.602761
CAACGCA231550.034.7772375
ATCAACG237300.034.0522083
TATCAAC256500.032.3513072
AACGCAG257650.031.9845626
TCAACGC265500.030.5782994
GTACTAG43500.029.514561
ACGCAGA277400.028.8945947
GTACATG282100.028.5739751
CGCAGAG285600.027.9331848
GTGGTAT38850.027.4787961
TACATGG289350.027.427322
ACATGGG282500.027.1894723
GCATCGT45850.025.7202598
GCAGAGT311700.025.0095319
CATGGGG222950.024.1847424
CTGTGCG56550.024.1800049
TAGTACT58250.023.7266794
TAGGCAT58850.023.7242475
Produced by FastQC (version 0.11.5)