FastQCFastQC Report
Thu 26 May 2016
ERR580144_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580144_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7109684
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161690.22742220329342344No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135380.190416339179069No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83480.11741731418724095No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107500.049.9479941
GTATCAA190950.040.0123561
CAACGCA225450.032.501975
ATCAACG228100.032.1667143
GTACTAG43350.030.5858441
TATCAAC248600.030.3563562
AACGCAG251750.029.6460826
TCAACGC255850.028.971184
GTGGTAT38450.027.3912641
GTACATG278800.026.9153821
ACGCAGA275300.026.580847
TACATGG285850.025.9235062
CGCAGAG282450.025.8245878
TAGTACT58850.025.7175314
CTGTGCG56350.025.2699539
ACATGGG283000.025.1958033
TAGGCAT58650.024.7630545
CTAACGC20150.024.4930733
CTAGTAC67200.023.5716343
TCTAACG21600.023.5096262
Produced by FastQC (version 0.11.5)