FastQCFastQC Report
Thu 26 May 2016
ERR580143_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580143_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3901313
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59860.15343552286114956No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47310.12126686579620757No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57550.049.118181
GTATCAA102600.040.7537421
CAACGCA120550.033.537215
ATCAACG121750.033.1294333
TATCAAC126350.032.376842
AACGCAG132050.031.1301486
TCAACGC132000.030.7001254
ACGCAGA147700.027.0686237
GTACATG180000.026.2607231
CGCAGAG153150.025.9826558
GTACTAG19600.025.9167611
TACATGG183900.025.3641532
GTGGTAT24000.025.2808441
ACATGGG184100.024.6160813
CATGGGG149950.023.1688674
GCAGAGT167750.023.1626349
ATGGGGG112350.023.0554815
GCATCGT18350.022.0180828
TGGTATC27600.021.9771352
TGGGGGG103300.021.5115836
Produced by FastQC (version 0.11.5)