FastQCFastQC Report
Thu 26 May 2016
ERR580143_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580143_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3901313
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52250.13392926945364292No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45820.11744763878212283No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57950.049.087281
GTATCAA106600.040.534551
CAACGCA122900.033.9238475
ATCAACG123250.033.8275153
TATCAAC131300.032.729772
TCAACGC133500.031.441124
AACGCAG134400.031.4371916
ACGCAGA154150.026.9215747
CGCAGAG157600.026.2427758
GTGGTAT24900.026.0583421
GTACATG180850.024.7765831
TACATGG184000.023.8665982
GTACTAG18900.023.3847681
GCAGAGT174450.023.3832159
ACATGGG186650.022.7905083
CATGGGG152400.021.0651024
TGGTATC28700.020.641922
TGGGGGG102300.020.1684346
ATGGGGG115800.019.8487725
CTAGTAC26800.019.8187873
Produced by FastQC (version 0.11.5)