FastQCFastQC Report
Thu 26 May 2016
ERR580142_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580142_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7957891
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124130.1559835388547041No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100090.1257745299602621No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87350.045.2733041
GTATCAA155950.038.7461
CAACGCA164500.034.833025
ATCAACG171600.033.3625143
GTACATG302700.033.3525241
TACATGG310250.032.1086242
ACATGGG303300.031.8004843
TATCAAC190100.031.3079132
AACGCAG187300.031.3038676
CATGGGG241450.030.194824
TCAACGC196250.029.5325764
ATGGGGG179500.029.1987155
GAGTACA201200.027.905221
ACGCAGA211800.026.9074427
TCTAACG19850.026.7621142
AGTACAT203650.026.7086222
TAGTACT61450.026.466844
CTAACGC20350.026.3311123
GTACTAG50100.026.2802471
TGGGGGG159800.025.9306246
Produced by FastQC (version 0.11.5)