FastQCFastQC Report
Thu 26 May 2016
ERR580142_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580142_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7957891
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113060.14207281803683916No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95200.11962968580494507No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85800.044.929961
GTATCAA148800.038.3236921
CAACGCA163450.033.1840485
GTACATG294950.032.515561
ATCAACG172900.031.370743
TACATGG302200.031.269972
ACATGGG299850.030.519393
GTACTAG47900.030.3272111
AACGCAG186850.029.9067976
TATCAAC189600.029.6068272
CATGGGG237550.028.6300414
TCAACGC194300.028.229724
ATGGGGG182750.027.493425
GAGTACA203050.027.4594081
TAGTACT62900.026.7510534
AGTACAT200700.026.4701822
TGGGGGG156900.026.359736
TAGGCAT60200.025.92095
CTAGGCA63700.025.8248064
CTAACGC20450.025.7417853
Produced by FastQC (version 0.11.5)