FastQCFastQC Report
Thu 26 May 2016
ERR580141_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580141_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9614031
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168370.1751294540240197No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120840.12569129431764886No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA120600.049.679361
GTATCAA234450.043.94871
CAACGCA271000.036.512745
ATCAACG274900.036.0097733
TATCAAC296500.034.2496572
AACGCAG306550.033.0424046
TCAACGC322950.030.8430464
ACGCAGA324800.030.3336527
CGCAGAG331350.029.591938
GTACATG367150.027.1291581
TACATGG371500.026.2468472
GCAGAGT369150.025.9160739
ACATGGG366300.025.7669813
GTACTAG60950.024.3047261
GCATCGT56200.023.4077428
CTGTGCG67650.023.1991989
CATGGGG281550.022.9900554
TAGGCAT77100.022.8023095
TAGTACT72600.021.8847544
ATGGGGG220800.021.6938535
Produced by FastQC (version 0.11.5)