FastQCFastQC Report
Thu 26 May 2016
ERR580141_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580141_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9614031
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150790.15684367982587116No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112960.1174949404677393No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA125350.047.3328441
GTATCAA226850.040.4961171
CAACGCA259650.034.049315
ATCAACG264050.033.5355033
TATCAAC285900.031.8865172
AACGCAG300050.030.3400826
TCAACGC312150.028.653874
ACGCAGA316950.027.7733177
CGCAGAG324150.026.9973538
GTACTAG59200.025.9689061
GTACATG364600.025.9570121
TACATGG371450.025.188142
ACATGGG364800.024.7504253
GCAGAGT365950.023.4118989
CATGGGG277800.023.0944844
TAGGCAT77550.022.7884035
GTGGTAT47700.022.2749961
ATGGGGG217300.021.71615
GCTATTG70550.020.6515278
TAGTACT72450.020.370384
Produced by FastQC (version 0.11.5)