FastQCFastQC Report
Thu 26 May 2016
ERR580140_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580140_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7960561
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232990.2926803776768999No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189660.23824954045324193No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112000.14069360187052143No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA133850.051.294561
GTATCAA241250.041.0903781
CAACGCA278250.034.526335
ATCAACG283100.033.9833533
TATCAAC301250.032.6315882
AACGCAG310250.031.5104586
TCAACGC317150.030.5578754
GTACTAG53550.029.5064321
GTGGTAT45050.028.914911
ACGCAGA333450.028.642757
CGCAGAG340850.027.9530438
GTACATG340250.027.9321921
TACATGG345750.027.0719932
ACATGGG344950.026.5412313
GCATCGT52150.026.4860678
CTGTGCG65950.025.2917549
TAGGCAT66400.025.1991925
GCAGAGT371550.025.1905389
CATGGGG267700.024.3867024
TAGTACT69800.024.3754314
Produced by FastQC (version 0.11.5)