FastQCFastQC Report
Thu 26 May 2016
ERR580140_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580140_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7960561
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203570.25572318332841115No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178060.22367770311665222No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105970.13311875884124247No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA135850.052.2267231
GTATCAA246550.040.829571
CAACGCA292750.033.2651565
ATCAACG293850.033.237853
TATCAAC315350.031.5955472
AACGCAG324750.030.6076976
GTACTAG53750.030.3538781
TCAACGC330200.029.805874
ACGCAGA352000.027.4905327
CGCAGAG358350.026.9638828
GTACATG346000.026.9061931
GTGGTAT48250.026.2130871
TACATGG351500.026.1923892
TAGGCAT67600.026.1419435
CTGTGCG69800.025.9207449
ACATGGG351900.025.297283
GCTATTG65250.025.2089218
TAGTACT73500.024.5553474
GCAGAGT391050.024.4193219
GGGCACC67000.024.05992394
Produced by FastQC (version 0.11.5)