FastQCFastQC Report
Thu 26 May 2016
ERR580139_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580139_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8076189
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243900.3019988759549832No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199550.24708436120056132No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119810.14834967334221624No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA134450.051.622881
GTATCAA243050.040.4746131
CAACGCA277400.033.9888535
ATCAACG283500.033.3234253
TATCAAC306100.031.8997692
GTACTAG62950.031.7475241
AACGCAG313550.031.1209856
TCAACGC326000.029.2397164
GTACATG325200.028.3269981
GCATCGT63700.028.3226748
ACGCAGA331650.028.2053227
GTGGTAT45750.027.8544671
CGCAGAG336150.027.744058
ACATGGG323400.027.3227563
TACATGG331950.027.3195722
TAGGCAT80600.027.2912145
CTGTGCG74650.026.7531099
CTAACGC30000.026.1638623
TAACGCC30050.026.12134
TCTAACG30650.025.615352
Produced by FastQC (version 0.11.5)