FastQCFastQC Report
Thu 26 May 2016
ERR580139_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580139_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8076189
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210780.26098943449688955No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183270.22692633864809256No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110480.136797194815525No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA138200.050.1812931
GTATCAA241400.038.934391
CAACGCA276200.032.7233285
GTACTAG61350.032.341171
ATCAACG281600.032.0799263
TATCAAC311100.029.8639642
AACGCAG312900.029.529836
GGGCACC72100.029.4630894
CTGTGCG80250.029.3392379
TAGGCAT77550.029.3336055
TAGTACT82850.028.5354924
TCAACGC322450.028.2780384
GTGGTAT45050.027.4485131
CTAGGCA85750.027.406014
ACGCAGA338050.026.5960467
GTACATG325250.026.511841
CGCAGAG345100.026.039268
TACATGG330700.025.7765122
ACATGGG323050.025.5340673
ACTGTGC103350.025.2387738
Produced by FastQC (version 0.11.5)