FastQCFastQC Report
Thu 26 May 2016
ERR580137_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580137_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3325480
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG44340.13333413522258442No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC42690.12837244548155455No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53050.045.646971
GTATCAA93150.039.7771341
CAACGCA104700.033.9897355
ATCAACG109050.032.551593
TATCAAC112950.032.429092
AACGCAG120500.030.5748446
TCAACGC119100.030.19914
GTACTAG18900.029.8544831
GTACATG205200.028.2308161
TACATGG208250.027.6137282
ACATGGG213900.026.4207153
ACGCAGA137500.025.939487
GTGGTAT20600.025.564711
CATGGGG177400.025.2041024
CGCAGAG142850.024.967628
ATGGGGG134350.024.9138535
TGGGGGG123000.023.5730236
TAGTACT25300.023.415014
GCATCGT22050.023.2291628
CTAGTAC26800.023.1574123
Produced by FastQC (version 0.11.5)