FastQCFastQC Report
Thu 26 May 2016
ERR580137_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580137_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3325480
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG42340.1273199658395179No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC39770.11959175818227745No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47700.045.089151
GTATCAA85250.040.388161
CAACGCA98500.033.455
ATCAACG100850.032.765243
TATCAAC105800.032.4293672
AACGCAG113300.029.7424986
TCAACGC111700.029.7079284
GTACTAG17900.028.9844631
GTACATG200500.028.0876621
TACATGG203400.027.4284342
ACATGGG211500.025.8688163
ACGCAGA130850.024.9990637
CATGGGG177600.024.8509794
CGCAGAG136300.023.8615388
TGGGGGG115450.023.8149746
ATGGGGG133000.023.7836075
GTGGTAT22900.023.4798321
GTAAGTA23250.022.2367342
TAGGCAT27350.022.1690315
TGGTATC22300.021.708692
Produced by FastQC (version 0.11.5)