FastQCFastQC Report
Thu 26 May 2016
ERR580135_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580135_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8823981
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222860.252561740556785No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172030.19495735541588316No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106010.12013851797731659No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA147000.049.2328761
GTATCAA270750.040.937851
CAACGCA302650.034.803325
ATCAACG309550.033.9802553
TATCAAC343100.032.022562
GTACTAG73600.031.3716681
AACGCAG349900.031.269856
GGGCACC82100.029.25763194
ACGCAGA357900.029.2461417
CGCAGAG363250.028.7251918
GTGGTAT44500.028.3210071
TCAACGC379950.027.9448594
TAGGCAT98700.027.3346965
GTACATG385050.027.222431
TATTCTC51600.026.7804055
TACATGG391350.026.4286772
ACATGGG384800.026.0771523
GCAGAGT392350.026.0480089
CTAGGCA106100.026.0479814
GCATCGT83950.025.9107698
Produced by FastQC (version 0.11.5)