FastQCFastQC Report
Thu 26 May 2016
ERR580135_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580135_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8823981
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197570.22390120740287178No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160740.18216267691419552No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100530.11392816915630258No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA146300.048.654681
GTATCAA268100.040.9655761
CAACGCA301000.034.7429125
ATCAACG308200.033.9775963
TATCAAC340300.032.0395772
AACGCAG349550.031.1115976
GTACTAG75650.029.769371
GTGGTAT37850.029.3149491
GGGCACC80700.028.94317694
ACGCAGA362700.028.6619577
TCAACGC377250.028.0698184
CGCAGAG370350.027.9936148
AGGGCTA92250.026.5444725
GTACATG385000.025.9502331
GCTATTG93550.025.8217188
TAGGCAT100100.025.636655
GCAGAGT401600.025.5564279
ATTTAGG97600.025.4347321
TACATGG389400.025.3675232
ACATGGG383200.025.0338483
Produced by FastQC (version 0.11.5)