FastQCFastQC Report
Thu 26 May 2016
ERR580134_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580134_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9230912
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288180.31219017145868144No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229820.24896781596444642No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144070.15607341939777997No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA134950.048.9301951
GTATCAA251450.042.4951551
CAACGCA296500.034.670395
ATCAACG301050.034.1668243
TATCAAC318900.033.1840632
AACGCAG329050.031.6807256
TCAACGC335150.030.8859654
GTACTAG58500.028.861661
GTACATG353600.028.556341
ACGCAGA359350.028.5519837
CGCAGAG370750.027.6482478
TACATGG361650.027.3106752
ACATGGG355700.026.8811593
GTGGTAT51850.025.6696761
CATGGGG287450.024.8201074
GCATCGT61500.024.6842828
CTGTGCG77550.024.665739
GCAGAGT414600.024.3492899
TAGTACT76700.024.0978094
ATGGGGG213650.023.3311565
Produced by FastQC (version 0.11.5)