FastQCFastQC Report
Thu 26 May 2016
ERR580134_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580134_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9230912
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252020.27301744399686617No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205560.22268655578127058No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138600.150147677715918No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA134750.049.1883131
GTATCAA244500.042.0476261
CAACGCA297900.033.494685
ATCAACG302000.033.0556953
TATCAAC319050.032.0118982
GTACTAG58550.031.9541871
AACGCAG332350.030.6012446
TCAACGC336250.029.982054
ACGCAGA362500.027.6152557
GTACATG330800.027.0139561
CGCAGAG377250.026.5355348
TACATGG335300.026.0729272
TAGGCAT76800.026.009025
TAGTACT74500.025.424074
GTGGTAT52000.025.402391
ACATGGG331100.025.3383143
CTGTGCG76050.024.5340239
CTAACGC24750.024.1172163
CTAGGCA84200.024.113934
GCAGAGT414600.023.8389689
Produced by FastQC (version 0.11.5)