FastQCFastQC Report
Thu 26 May 2016
ERR580133_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580133_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8957145
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117800.13151512005220414No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94000.10494415352213234No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107600.038.8043751
GTATCAA178950.035.182681
GTACTAG70900.034.3529131
GTACATG354050.033.8122331
TACATGG363200.032.4874532
CAACGCA180900.032.427275
ACATGGG358550.032.103443
ATCAACG189600.031.0369113
CATGGGG274650.029.863824
GCATCGT73250.029.8255168
CTAACGC31750.029.6072983
TCTAACG32350.029.208522
TAGGCAT88750.029.0762465
GAGTACA235500.029.0503981
ATGGGGG214300.028.6892995
TAACGCC32950.028.6728044
AGTACAT233800.028.149542
AACGCAG216500.027.9505846
TATCAAC221650.027.9322432
CTAGGCA97200.027.2735924
Produced by FastQC (version 0.11.5)