FastQCFastQC Report
Thu 26 May 2016
ERR580133_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580133_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8957145
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103860.11595212537030494No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106800.040.1504861
GTATCAA173150.034.2963681
GTACTAG69450.033.3088871
GTACATG348700.032.8467641
TACATGG355200.031.8882792
CAACGCA175450.031.5024385
ACATGGG350550.031.3470653
TCTAACG31050.030.1341342
ATCAACG185400.029.8126033
CTAACGC31250.029.7795073
TAACGCC31300.029.5811144
CTGTGCG87700.028.8830499
CATGGGG273150.028.8036084
TAGGCAT88050.028.717275
TAGTACT89000.028.1996614
ATGGGGG208150.028.0437055
GAGTACA239500.027.7594681
AACGCAG213050.027.0001286
TGGGGGG180000.026.9969086
TATCAAC218000.026.9169182
Produced by FastQC (version 0.11.5)