FastQCFastQC Report
Thu 26 May 2016
ERR580132_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580132_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8014778
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117190.1462173999080199No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91140.11371494007694288No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86350.041.9901351
GTATCAA148300.038.497521
CAACGCA160300.033.751115
ATCAACG167850.032.3717773
GTACATG266650.032.2219771
TACATGG270700.031.1941322
ACATGGG267900.030.7391153
GTACTAG42500.030.4297921
TATCAAC184500.030.4272562
AACGCAG183950.030.0371366
TCAACGC188000.029.002874
CATGGGG207000.028.6342354
ACGCAGA204000.026.579767
TCTAACG20700.025.8934122
ATGGGGG154800.025.7800055
GAGTACA183550.025.3906881
CGCAGAG213500.025.3748878
TAACGCC20750.025.3711854
CTAACGC20950.025.1283513
GCATCGT46100.025.0730328
Produced by FastQC (version 0.11.5)